Advances in Single-Cell Sequencing for Infectious Diseases: Progress and Perspectives.

IF 14.3 1区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Mengyuan Lyu, Yang Liu, Jian Zhou, Hongli Lai, Hongxia Ruan, Dongsheng Wu, Shun Zhu, Xudong Zhou, Wananqi Ma, Yuchen Huang, Shuting Lei, Han Luo, Jie Chen, Binwu Ying
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引用次数: 0

Abstract

Infectious diseases are a persistent threat throughout the twenty-first century, owing to their widespread transmission mediated by global connectivity. Benefiting from the advancement of eukaryotic, prokaryotic (e.g., MATQ-seq and BacDrop), and dual (e.g., PatH-Cap) single-cell sequencing technologies, changes in each side and host-pathogen interactions can be elucidated. Pathogens undergo adaptive evolution in the host microenvironment, exhibiting different transcription profiles (e.g., upregulated HSV-1 immediate-early genes during infection), transcription efficiencies, and others. Consequently, they develop distinct replication capacity, virulence, and other phenotypes. For the host, intrinsic differences among cells and variations brought by diverse phenotypic pathogens together determine the heterogeneity of its responses (e.g., the dual roles of interferon-I at different infection stages). Interferon-related pathway alterations (influencing both adaptive and innate immune responses) and cell exhaustion (manifesting as the functional impairment of cells) are observed in various infections, seemingly as a shared approach in host-pathogen interactions. These underlying commonalities provide a basis for the development of broad-spectrum therapeutic targets. Collectively, this work reviewed recent progress of single-cell sequencing technologies and their applications in infectious diseases, aiming to facilitate the development of powerful diagnostic or therapeutic strategies and thus advance precision medicine.

传染病单细胞测序研究进展与展望
传染病在全球连通性的推动下广泛传播,是整个二十一世纪的持续威胁。得益于真核生物、原核生物(如MATQ-seq和BacDrop)和双单细胞测序技术的进步,可以阐明每侧的变化和宿主-病原体相互作用。病原体在宿主微环境中经历适应性进化,表现出不同的转录谱(例如,感染期间HSV-1立即早期基因上调)、转录效率等。因此,它们发展出不同的复制能力、毒力和其他表型。对于宿主来说,细胞之间的内在差异和不同表型病原体带来的变异共同决定了其反应的异质性(例如,干扰素- 1在不同感染阶段的双重作用)。干扰素相关通路改变(影响适应性和先天免疫反应)和细胞耗竭(表现为细胞功能损伤)在各种感染中被观察到,似乎是宿主-病原体相互作用的共同途径。这些潜在的共性为开发广谱治疗靶点提供了基础。综上所述,本文综述了单细胞测序技术及其在传染病中的应用的最新进展,旨在促进强大的诊断或治疗策略的发展,从而推进精准医学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Science
Advanced Science CHEMISTRY, MULTIDISCIPLINARYNANOSCIENCE &-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
18.90
自引率
2.60%
发文量
1602
审稿时长
1.9 months
期刊介绍: Advanced Science is a prestigious open access journal that focuses on interdisciplinary research in materials science, physics, chemistry, medical and life sciences, and engineering. The journal aims to promote cutting-edge research by employing a rigorous and impartial review process. It is committed to presenting research articles with the highest quality production standards, ensuring maximum accessibility of top scientific findings. With its vibrant and innovative publication platform, Advanced Science seeks to revolutionize the dissemination and organization of scientific knowledge.
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