Mengyuan Lyu, Yang Liu, Jian Zhou, Hongli Lai, Hongxia Ruan, Dongsheng Wu, Shun Zhu, Xudong Zhou, Wananqi Ma, Yuchen Huang, Shuting Lei, Han Luo, Jie Chen, Binwu Ying
{"title":"Advances in Single-Cell Sequencing for Infectious Diseases: Progress and Perspectives.","authors":"Mengyuan Lyu, Yang Liu, Jian Zhou, Hongli Lai, Hongxia Ruan, Dongsheng Wu, Shun Zhu, Xudong Zhou, Wananqi Ma, Yuchen Huang, Shuting Lei, Han Luo, Jie Chen, Binwu Ying","doi":"10.1002/advs.202415678","DOIUrl":null,"url":null,"abstract":"<p><p>Infectious diseases are a persistent threat throughout the twenty-first century, owing to their widespread transmission mediated by global connectivity. Benefiting from the advancement of eukaryotic, prokaryotic (e.g., MATQ-seq and BacDrop), and dual (e.g., PatH-Cap) single-cell sequencing technologies, changes in each side and host-pathogen interactions can be elucidated. Pathogens undergo adaptive evolution in the host microenvironment, exhibiting different transcription profiles (e.g., upregulated HSV-1 immediate-early genes during infection), transcription efficiencies, and others. Consequently, they develop distinct replication capacity, virulence, and other phenotypes. For the host, intrinsic differences among cells and variations brought by diverse phenotypic pathogens together determine the heterogeneity of its responses (e.g., the dual roles of interferon-I at different infection stages). Interferon-related pathway alterations (influencing both adaptive and innate immune responses) and cell exhaustion (manifesting as the functional impairment of cells) are observed in various infections, seemingly as a shared approach in host-pathogen interactions. These underlying commonalities provide a basis for the development of broad-spectrum therapeutic targets. Collectively, this work reviewed recent progress of single-cell sequencing technologies and their applications in infectious diseases, aiming to facilitate the development of powerful diagnostic or therapeutic strategies and thus advance precision medicine.</p>","PeriodicalId":117,"journal":{"name":"Advanced Science","volume":" ","pages":"e15678"},"PeriodicalIF":14.3000,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Science","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1002/advs.202415678","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Infectious diseases are a persistent threat throughout the twenty-first century, owing to their widespread transmission mediated by global connectivity. Benefiting from the advancement of eukaryotic, prokaryotic (e.g., MATQ-seq and BacDrop), and dual (e.g., PatH-Cap) single-cell sequencing technologies, changes in each side and host-pathogen interactions can be elucidated. Pathogens undergo adaptive evolution in the host microenvironment, exhibiting different transcription profiles (e.g., upregulated HSV-1 immediate-early genes during infection), transcription efficiencies, and others. Consequently, they develop distinct replication capacity, virulence, and other phenotypes. For the host, intrinsic differences among cells and variations brought by diverse phenotypic pathogens together determine the heterogeneity of its responses (e.g., the dual roles of interferon-I at different infection stages). Interferon-related pathway alterations (influencing both adaptive and innate immune responses) and cell exhaustion (manifesting as the functional impairment of cells) are observed in various infections, seemingly as a shared approach in host-pathogen interactions. These underlying commonalities provide a basis for the development of broad-spectrum therapeutic targets. Collectively, this work reviewed recent progress of single-cell sequencing technologies and their applications in infectious diseases, aiming to facilitate the development of powerful diagnostic or therapeutic strategies and thus advance precision medicine.
期刊介绍:
Advanced Science is a prestigious open access journal that focuses on interdisciplinary research in materials science, physics, chemistry, medical and life sciences, and engineering. The journal aims to promote cutting-edge research by employing a rigorous and impartial review process. It is committed to presenting research articles with the highest quality production standards, ensuring maximum accessibility of top scientific findings. With its vibrant and innovative publication platform, Advanced Science seeks to revolutionize the dissemination and organization of scientific knowledge.