Universal Artificial Urinary Biomarker Probe Enabled by an Aptamer-DNAzyme-Nanozyme Construct.

IF 9.6 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-07-16 Epub Date: 2025-07-04 DOI:10.1021/acs.nanolett.5c02356

Yi Xiao, Jia Hui Wong, Nan Gao, Anna M Barron, Chelsea Violita Stanley, Aung Than, Lewen Zheng, Tong Ling, Dong-Sheng Li, Peng Chen

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引用次数: 0

Abstract

Artificial urinary biomarker probes (AUBPs) have recently emerged as a new class of diagnostic tools. However, they are applicable only to the biomarkers with catalytic activities or high reactivities. Here, we present a general modular design of AUBPs, integrating an aptamer for target recognition, DNAzyme for triggerable signal transduction, and nanozyme as a urinary artificial biomarker. For proof-of-concept demonstration, we developed an AUBP that is specifically and sensitively responsive to circulating an amyloid-β oligomer (AβO). After the nanoprobe was injected into circulation, colorimetric urinalysis was conducted to infer the circulating level of AβO for early diagnosis of Alzheimer's disease (AD). This approach can unambiguously distinguish AD mice from healthy ones, achieving a low detection limit of ∼2.8 pg/mL. It is ∼67 times more sensitive than the conventional enzyme-linked immunosorbent assay, owing to the cascaded signal amplification enabled by DNAzyme activity, enrichment by renal filtration, and chromogenic reaction catalyzed by nanozyme.

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适体- dnazyme -纳米酶构建的通用人工尿液生物标志物探针。

人工尿液生物标志物探针（aubp）是近年来出现的一类新的诊断工具。然而，它们仅适用于具有催化活性或高反应性的生物标志物。在这里，我们提出了aubp的通用模块化设计，整合了用于目标识别的适体，用于触发信号转导的DNAzyme和作为尿液人工生物标志物的纳米酶。为了验证概念，我们开发了一种AUBP，它对循环中的淀粉样蛋白-β低聚物（AβO）具有特异性和敏感性。将纳米探针注入血液循环后，进行尿比色分析，推断AβO的循环水平，用于阿尔茨海默病（AD）的早期诊断。该方法可以明确区分AD小鼠和健康小鼠，达到2.8 pg/mL的低检测限。由于DNAzyme活性的级联信号放大、肾滤过的富集和纳米酶催化的显色反应，它的灵敏度比传统的酶联免疫吸附法高67倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.