Comprehensive analysis of the gut microbial diversity and metabolic profiles of brown bears and black bears from the Qinghai-Xizang Plateau

IF 1.8 3区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal genetics Pub Date : 2025-07-05 DOI:10.1111/age.70028
Shiyin Zhong, Runjin Wang, Lanting Wei, Jie Zhang, Jing Feng, Zhuoga Zhaxi, Zhongchao Su, Zhimin Zhang, Qiongli Zhou, Jirui Yang, Hui Wang
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引用次数: 0

Abstract

Brown bears (Ursus arctos) and black bears (Ursus thibetanus) are important species on the Qinghai-Xizang Plateau. However, limited studies have been conducted on these species due to sampling constraints. This study, using 16S rRNA amplicon sequencing and untargeted metabolomics, analyzed fecal samples from six wild brown bears and six wild black bears. The results revealed distinct gut microbiota profiles, with brown bears showing higher microbial richness, particularly in Proteobacteria, Bacteroidota, and Actinobacteriota, while black bears were mainly enriched in Firmicutes. Lipid metabolism emerged as the primary metabolic pathway for both species, probably aiding adaptation to the plateau environment. Notably, we found positive correlations between differential metabolites and specific microbiota; in addition, traces of pesticides suggested contamination in the region. Our study provides valuable insights into the gut microbial diversity and metabolomic features of wild brown bears and black bears from the Qinghai-Xizang Plateau, contributing to our understanding of wildlife ecology in this unique environment.

青藏高原棕熊和黑熊肠道微生物多样性及代谢特征的综合分析
棕熊(Ursus arctos)和黑熊(Ursus thibetanus)是青藏高原的重要物种。然而,由于采样的限制,对这些物种的研究有限。本研究采用16S rRNA扩增子测序和非靶向代谢组学,分析了6只野生棕熊和6只野生黑熊的粪便样本。结果显示了不同的肠道微生物群分布,棕熊的肠道微生物丰富度更高,特别是变形菌门、拟杆菌门和放线菌门,而黑熊主要富集于厚壁菌门。脂质代谢是这两个物种的主要代谢途径,可能有助于适应高原环境。值得注意的是,我们发现差异代谢物与特定微生物群之间呈正相关;此外,残留的农药表明该地区受到了污染。我们的研究为青藏高原野生棕熊和黑熊的肠道微生物多样性和代谢组学特征提供了有价值的见解,有助于我们了解这一独特环境下的野生动物生态学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal genetics
Animal genetics 生物-奶制品与动物科学
CiteScore
4.60
自引率
4.20%
发文量
115
审稿时长
5 months
期刊介绍: Animal Genetics reports frontline research on immunogenetics, molecular genetics and functional genomics of economically important and domesticated animals. Publications include the study of variability at gene and protein levels, mapping of genes, traits and QTLs, associations between genes and traits, genetic diversity, and characterization of gene or protein expression and control related to phenotypic or genetic variation. The journal publishes full-length articles, short communications and brief notes, as well as commissioned and submitted mini-reviews on issues of interest to Animal Genetics readers.
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