Chemical Derivatization-Based Liquid Chromatography-Mass Spectrometry Method for Fatty Acid Profiling in Biological Samples

IF 2.8 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Journal of separation science Pub Date : 2025-07-04 DOI:10.1002/jssc.70211

Jingxuan Yang, Yi Wu, Liang Zhao, Meng Li, Wenjun Guo, Yajuan Xu, Yang Wang

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引用次数: 0

Abstract

Fatty acids (FAs) are important metabolites for various biochemical functions in living organisms, including energy storage, cell structure, and cell signaling. Changes in FAs composition were significantly related to abnormal metabolic levels and body status. In this study, the derivatization-based liquid chromatography-quadrupole-orbitrap mass spectrometry method was established for FA analysis under parallel reaction monitoring mode. The 4-amino-1-benzylpiperidine (4A1BP) was used as a derivatization reagent to amide with FAs. The results showed that protonated molecules of FA+4A1BP–H₂O were observed, and characteristic fragment ions at m/z 174.1280 and 91.0548 were detected in the tandem mass spectrum. The derivatization method was optimized in terms of sample preparation, chromatographic separation, and mass spectrometric conditions. The linearity, stability, and repeatability of the method were also investigated with good results. Finally, we applied the established method to analyze the serum and liver tissue samples, successfully evaluating the efficacy of Qizha Shuangye granules in the treatment of hyperlipidemia rats. The established method has broad utility and great potential in the detection of FAs, which can provide technical support for disease mechanism research, biomarker discovery, as well as food quality assurance, and nutritional value evaluation.

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基于化学衍生的液相色谱-质谱法分析生物样品中的脂肪酸

脂肪酸（FAs）是生物体内各种生化功能的重要代谢物，包括能量储存、细胞结构和细胞信号传导。FAs组成的变化与代谢水平异常和身体状态显著相关。本研究在平行反应监测模式下，建立了衍生化液相色谱-四极轨道阱质谱分析FA的方法。以4-氨基-1-苄基哌啶（4A1BP）为衍生化试剂与FAs酰胺化。结果表明，FA+ 4A1BP-H2O存在质子化分子，在串联质谱中检测到m/z为174.1280和91.0548的特征片段离子。从样品制备、色谱分离和质谱条件三个方面对衍生化法进行了优化。并对该方法的线性、稳定性和重复性进行了考察，结果良好。最后，应用建立的方法对血清和肝组织样本进行分析，成功评价芪扎双叶颗粒对高脂血症大鼠的治疗效果。所建立的方法在FAs检测方面具有广泛的实用性和巨大的潜力，可为疾病机理研究、生物标志物发现以及食品质量保证和营养价值评价提供技术支持。

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来源期刊

Journal of separation science 化学-分析化学

CiteScore

6.30

自引率

16.10%

发文量

408

审稿时长

1.8 months

期刊介绍： The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.