Multi-step infrared macro-fingerprint features and lipid metabolism in HepG2 cells: Intervention of different sourced phospholipids

Applied Food Research Pub Date : 2025-06-30 DOI:10.1016/j.afres.2025.101125

Yulong Zhao , Xutao Li , Guihong Luo , Ryosuke Matsuoka , Yimeng Zhao , Qingqing Jiang , Long Zhang , Wenzheng Shi , Xichang Wang , Mingyu Yin

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Abstract

This study examined the efficacy of capelin roe phospholipids (CPL), egg yolk phospholipids (EPL), and soybean phospholipids (SPL) on lipid metabolism disorders induced by oleic acid in HepG2 cells. The results showed that OA led to an increase in intracellular triglycerides, cholesterol, malondialdehyde, carbohydrates, nucleic acids, -CH₂-, ν(C=O)/ν_as (CH₂+CH₃), and a decrease in cellular protein content, from which CPL, EPL, and SPL significantly inhibited these effects (P < 0.05). It was revealed that CPL, EPL, and SPL treatments inhibited oxidative stress and lipid peroxidation in cells while safeguarding intracellular nucleic acid structures to maintain normal energy metabolic pathways, based on FT-IR spectroscopy and chemometric analysis. This study highlights the potential direction for further development of phospholipid extraction and the creation of phospholipid-based functionalized products.

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HepG2细胞多步红外宏观指纹特征与脂质代谢：不同来源磷脂的干预

本实验研究了毛鳞鱼子磷脂（CPL）、蛋黄磷脂（EPL）和大豆磷脂（SPL）对油酸诱导的HepG2细胞脂质代谢紊乱的影响。结果表明，OA导致细胞内甘油三酯、胆固醇、丙二醛、碳水化合物、核酸、-CH2-、ν(C=O)/ν (CH2+CH3)升高，细胞蛋白质含量降低，CPL、EPL和SPL显著抑制了这些影响(P <；0.05)。FT-IR光谱和化学计量学分析显示，CPL、EPL和SPL处理可抑制细胞氧化应激和脂质过氧化，同时保护细胞内核酸结构，维持正常的能量代谢途径。本研究为进一步发展磷脂提取和制备磷脂基功能化产物指明了可能的方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Food Research

CiteScore

4.50

自引率

0.00%

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