Molecular detection of Dientamoeba fragilis in children in southern Xinjiang, China

IF 2.9 Q2 PARASITOLOGY

Food and Waterborne Parasitology Pub Date : 2025-07-04 DOI:10.1016/j.fawpar.2025.e00275

Yafei Zhao , Wenxuan Ma , Duoduo Su , Zhenjie Zhang , Aiyun Zhao , Fuchang Yu , Meng Qi

引用次数: 0

Abstract

Dientamoeba fragilis is a common intestinal parasite in human and animals worldwide. In this study, 609 fecal samples were collected from preschool children in 11 counties in Southern Xinjiang, China. All samples were screened for D. fragilis using PCR targeting SSU rRNA gene, revealing an infection rate of 4.4 % (27/609). Seven of the 11 counties were D. fragilis-positive. The highest infection rate was 15.9 % (10/63) in Yopurga, and the lowest infection rate was 0.9 % (1/109) in Lop. Infection rates in boys and girls were 4.4 % (13/299) and 4.5 % (14/310), respectively. Genetic analysis identified all 27 positive samples as genotype 1. These results confirmed the presence of D. fragilis in children in southern Xinjiang, China. The high degree of sequence homology in the SSU rRNA gene indicates a clonal distribution pattern for D. fragilis.

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新疆南部地区儿童脆弱地形虫的分子检测

脆弱地阿米巴是世界范围内人类和动物常见的肠道寄生虫。本研究收集了南疆11个县学龄前儿童的609份粪便样本。所有样本均采用靶向SSU rRNA基因的PCR方法筛选脆弱单胞菌，感染率为4.4%（27/609）。11个县中有7个县呈脆弱杆菌阳性。尤普加省感染率最高，为15.9%(10/63)，罗布县感染率最低，为0.9%（1/109）。男童和女童感染率分别为4.4%（13/299）和4.5%（14/310）。遗传分析鉴定27份阳性样本均为基因1型。这些结果证实了脆弱梭菌在中国南疆儿童中的存在。SSU rRNA基因的高度同源性表明脆弱丝裂菌的克隆分布模式。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.