Acute effects of heat intervention and hybrid exercise on protein synthesis, ribosome biogenesis and autophagy

IF 2.9 2区 生物学 Q2 BIOLOGY
Tom Normand-Gravier , Robert Solsona , Flavie Arnould , Roméo Deriaz , Christelle Bertrand-Gaday , Fabio Borrani , Henri Bernardi , Anthony M.J. Sanchez
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Abstract

The use of kaumatherapy (i.e. heat exposure like sauna-bathing) as passive intervention has become of growing interest to promote skeletal muscle adaptations such as strength gains and preservation of muscle mass. Importantly, the effects of a single exposure to heat (HE) in combination with hybrid exercises, designed to induce both aerobic and resistance adaptations (EX), on protein turnover remains unclear. The objective of this investigation was to evaluate the responses to HE, EX and their combination (EX + HE) on the expression of mRNA and protein related to proteosynthesis, ribosome biogenesis and content, as well as autophagy and cellular stress pathways. Eight-week-old male mice C57BL/6 J mice (n = 8 per condition) underwent acute HE (45min, 40 °C), EX (high-intensity inclined treadmill running) or EX + HE immediately after exercise. Mice were euthanized 240 min post-interventions and quadriceps muscles were harvested for analysis. Acute HE increased the mRNA expression of markers of ribosome biogenesis (POL1RA, UBF), but did not enhance ribosomal content (rRNA 18 S and 28 S) nor ribosomal transcription (pre-rRNA 45 S). Concerning the modulation of protein synthesis, EX induced an increase of MTORC1 downstream targets (P-S6K1, P-RPS6) and protein synthesis flux (assessed by puromycin incorporation) while HE alone did not and post-exercise HE had no additional effects. EX + HE also led to enhanced protein synthesis rates, but did not confer any additional benefit compared to EX alone. Finally, only EX + HE increased the autophagy flux marker LC3-B II/I, the microautophagy marker LAMP2A and phosphorylation level of P-NFκB Ser536. However, no changes in protein carbonylation were detected at this time point. These results suggest that acute post-exercise HE has no additional effects on protein synthesis at 4 h post-exercise but, when combined with EX, increases autophagy and P-NFκB Ser536, suggesting a heightened cellular stress response.
热干预和混合运动对蛋白质合成、核糖体生物发生和自噬的急性影响
使用桑拿疗法(即热暴露,如桑拿浴)作为被动干预,促进骨骼肌适应,如力量增加和肌肉质量的保存,已成为越来越受关注的问题。重要的是,单次暴露于热(HE)结合混合运动(旨在诱导有氧和阻力适应(EX))对蛋白质周转的影响尚不清楚。本研究的目的是评估HE、EX及其组合(EX + HE)对蛋白质合成、核糖体生物发生和含量、自噬和细胞应激途径相关mRNA和蛋白表达的影响。8周龄雄性小鼠C57BL/6 J小鼠(每种情况n = 8只)在运动后立即进行急性HE(45分钟,40°C)、EX(高强度倾斜跑步机)或EX + HE。干预后240分钟对小鼠实施安乐死,收集股四头肌进行分析。急性HE增加了核糖体生物发生标志物(POL1RA, UBF) mRNA的表达,但没有增加核糖体含量(rRNA 18s和28s)和核糖体转录(pre-rRNA 45s)。在蛋白质合成的调节方面,EX诱导MTORC1下游靶标(P-S6K1, P-RPS6)和蛋白质合成通量(通过puromycin掺入评估)的增加,而HE单独没有,运动后HE没有额外的影响。EX + HE也能提高蛋白质合成率,但与单独使用EX相比,没有任何额外的益处。最后,只有EX + HE增加了自噬通量标志物LC3-B II/I、微自噬标志物LAMP2A和P-NFκB Ser536的磷酸化水平。然而,在这个时间点没有检测到蛋白质羰基化的变化。这些结果表明,急性运动后HE对运动后4小时的蛋白质合成没有额外的影响,但当与EX联合使用时,自噬和P-NFκB Ser536增加,表明细胞应激反应增强。
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来源期刊
Journal of thermal biology
Journal of thermal biology 生物-动物学
CiteScore
5.30
自引率
7.40%
发文量
196
审稿时长
14.5 weeks
期刊介绍: The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles
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