First detection of CTX-M-65 ESBL gene in Morocco: Evidence of transcontinental transmission linked to commercial exchange.

IF 3.3 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-01 Epub Date: 2025-06-30 DOI:10.1016/j.micpath.2025.107855

Oumaima Fazza, Bouchra Karraouane, Germán Kopprio, Brahim Bouchrif, Houda Ennassiri, Mireille Favard Ennachachibi, Abdelaziz Hmyene

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引用次数: 0

Abstract

Introduction: The study aimed to determine the occurrence of genes encoding antibacterial resistance in Escherichia coli (E. coli) isolates recovered from poultry products.

Method: ology: Antibiotic susceptibility was tested using the disc diffusion method, and then resistance genes and virulence profiles were determined by whole-genome analysis.

Results: Out of 154 poultry products, 96 (62.34 %) were E. coli. Two isolates were positive to the test of synergy between the clavulanic acid and the third generation of cephalosporin (C3G) and showed a high level of resistance to penicillins and fluoroquinolones. Besides, the isolate E2 was notably resistant to tobramycine, tigecycline, aztreonam, and chloramphenicol. Whole-genome screening showed the presence of ESBL genes, namely, blaCTX-M-65, blaEC-15, blaTEM-1, and quinolone resistance genes gyrA_S83L and parC_S80I, in addition to other resistance genes. The virulence profile did not show the presence of either of the Shiga toxin genes stx1 and sxt2; nevertheless, other virulence factors were detected, such as cvaC; responsible for the production of colicin V and iroABCDEN genes.

Conclusion: The results alert us about the hygienic state of poultry products and the increasing level of antibacterial resistance, and pose a potential risk that could be caused by the transmission of the antimicrobial resistance to humans through the consumption of food products, leading to a reduction of the available antibiotic treatment options.

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摩洛哥首次检测到CTX-M-65 ESBL基因：与商业交换有关的跨大陆传播证据。

前言：本研究旨在确定从禽类产品中分离的大肠杆菌（E. coli）中编码抗菌耐药基因的发生情况。方法：采用圆盘扩散法检测药敏，全基因组法检测耐药基因及毒力谱。结果：154件禽制品中，检出大肠杆菌96件（62.34%）；两株菌株对克拉维酸与第三代头孢菌素（C3G）协同作用试验呈阳性，对青霉素类和氟喹诺酮类药物具有较高的耐药性。此外，分离株E2对妥布霉素、替加环素、氨曲南和氯霉素均有明显耐药。全基因组筛选结果显示，除其他耐药基因外，还存在ESBL基因blaCTX-M-65、blaEC-15、blaem -1和喹诺酮类耐药基因gyrA_S83L和parC_S80I。毒力谱未显示志贺毒素基因stx1和sxt2的存在；然而，检测到其他毒力因子，如cvaC；负责大肠杆菌素V和iroABCDEN基因的产生。结论：该结果警示我们家禽产品的卫生状况和不断增加的抗菌药物耐药性水平，并提出了可能通过食用食品将抗菌药物耐药性传播给人类的潜在风险，导致可用的抗生素治疗方案减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)