Drug Resistance and Epidemiological Analysis of Carbapenem-Resistant Enterobacteriaceae in a Brain and Psychiatric Hospital of South China.

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-07-03 DOI:10.1002/jcla.70073

Lei Yue, Xiumei Zhu, Yuyan Kuang, Yulong Lin, Haiying Liu

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引用次数: 0

Abstract

Background: Carbapenem-resistant Enterobacteriaceae (CRE) has increasingly become a global health problem, and the drug resistance and epidemiology of its infection are important for disease prevention and control.

Methods: The CRE isolates detected in the hospital from July 2021 to April 2023 were collected, then the antibiotic sensitivity was detected by an automatic microbial analyzer. Polymerase chain reaction (PCR) was performed to detect carbapenem-resistance genes. The homology and epidemiological characteristics of the strains of Enterobacteriaceae were analyzed by repeated common sequence PCR(ERIC-PCR).

Results: A total of 94 non-repeating CRE isolates were collected mainly from the respiratory tract (67.02%) and urinary tract (22.34%). Among these, 73 strains (77.66%) were carbapenem-resistant Klebsiella pneumoniae (CRKP), while 21 strains (22.34%) were carbapenem-resistant Escherichia coli (CREC). Antibiotic susceptibility testing revealed a low resistance rate to tigecycline (2.13%). Analysis of CRE resistance genes demonstrated that blaKPC accounted for 93.15% of CRKP isolates (68/73), while blaNDM was present in 90.48% of CREC isolates (19/21). In the homology analysis, CRKP isolates were divided into five clusters with C_KP type representing the majority at 80.82% prevalence (59/73). Similarly, CREC could be classified into four clusters with A_EC type being predominant at 80.95% among the isolated strains (17/21). The detection of CRE strains involved multiple departments in two separate wards of this hospital, located approximately 28 km from each other.

Conclusions: CRE infection in this hospital has high homology and wide distribution; it is necessary to strengthen prevention and control measures to control CRE nosocomial infection spread.

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华南某脑精神病医院耐碳青霉烯肠杆菌科耐药及流行病学分析

背景：碳青霉烯耐药肠杆菌科（CRE）已日益成为全球性的健康问题，其感染的耐药性和流行病学对疾病的预防和控制具有重要意义。方法：收集2021年7月至2023年4月在医院检测到的CRE分离株，采用全自动微生物分析仪进行抗生素敏感性检测。采用聚合酶链反应（PCR）检测碳青霉烯耐药基因。采用重复共序列PCR（ERIC-PCR）分析了各菌株的同源性和流行病学特征。结果：94株非重复CRE分离株主要来自呼吸道（67.02%）和泌尿道（22.34%）。其中耐碳青霉烯肺炎克雷伯菌（CRKP） 73株（77.66%），耐碳青霉烯大肠埃希菌（CREC） 21株（22.34%）。药敏试验显示对替加环素耐药率低（2.13%）。CRE耐药基因分析显示，CRKP菌株中blaKPC占93.15% (68/73)，CREC菌株中blaNDM占90.48%（19/21）。在同源性分析中，CRKP分离株被分为5个聚类，其中CKP型占多数，患病率为80.82%（59/73）。同样，CREC可分为4个聚集型，其中AEC型占80.95%（17/21）。CRE菌株的检测涉及该医院两个独立病房的多个科室，彼此相距约28公里。结论：该院CRE感染同源性高，分布广泛；加强预防和控制措施，控制CRE院内感染的传播。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.