{"title":"Novel Role of Human Epicardial Adipocyte-Derived SPARCL1 in Postoperative Atrial Fibrillation Following Cardiovascular Surgery.","authors":"Taisuke Harada, Hidekazu Kondo, Masaki Takahashi, Hirochika Yamasaki, Masayuki Takano, Hiroki Sato, Yumi Ishii, Ichitaro Abe, Akira Fukui, Hidefumi Akioka, Tetsuji Shinohara, Yasushi Teshima, Kunio Yufu, Tetsuya Uemura, Kyoko Kawano, Shigeru Naono, Kazuhiro Ueno, Kenshi Yoshimura, Takayuki Kawashima, Takashi Shuto, Tomoyuki Wada, Hidenori Sako, Hirofumi Anai, Shinji Miyamoto, Naohiko Takahashi","doi":"10.1016/j.jacep.2025.05.008","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Postoperative atrial fibrillation (POAF) is associated with short- and long-term morbidity and mortality. However, the ability of the epicardial adipocyte to regulate POAF remains poorly understood.</p><p><strong>Objectives: </strong>The authors investigated the possible role of human epicardial adipocyte-derived adipokines in the regulation of the myocardial redox state and POAF.</p><p><strong>Methods: </strong>The authors prospectively studied 274 patients who underwent scheduled open-heart surgery. Finally, 149 patients without known atrial fibrillation were enrolled and divided into POAF and non-POAF groups. Epicardial adipose tissue biopsies and isolated preadipocytes were used to explore the important secretable adipokines associated with POAF.</p><p><strong>Results: </strong>POAF occurred in 53 (35.6%) patients. Genome-wide expression profiling of background-matched differentiated preadipocytes representing the POAF or non-POAF groups and quantitative polymerase chain reaction validation as a biological replicate using all cohort samples revealed that only SPARCL1 was significantly downregulated in the POAF group. SPARCL1 messenger RNA expression in differentiated preadipocytes correlated with SPARCL1 protein concentration in the preadipocyte culture medium. SPARCL1 attenuated angiotensin II-induced oxidative stress in neonatal rat myocytes. Coculture of human induced pluripotent stem cell-derived atrial cardiomyocyte with the differentiated preadipocyte revealed that the preadipocytes derived from non-POAF patients suppressed myocardial oxidative stress. Low SPARCL1 expression in human epicardial differentiated preadipocytes was associated with a higher risk of long-term adverse cardiovascular events after surgery.</p><p><strong>Conclusions: </strong>These findings suggested that the SPARCL1, derived from epicardial differentiated preadipocytes, may be an important paracrine adipokine that regulates POAF and subsequent cardiovascular outcomes by improving myocardial redox state.</p>","PeriodicalId":14573,"journal":{"name":"JACC. Clinical electrophysiology","volume":" ","pages":""},"PeriodicalIF":7.7000,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"JACC. Clinical electrophysiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jacep.2025.05.008","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CARDIAC & CARDIOVASCULAR SYSTEMS","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Postoperative atrial fibrillation (POAF) is associated with short- and long-term morbidity and mortality. However, the ability of the epicardial adipocyte to regulate POAF remains poorly understood.
Objectives: The authors investigated the possible role of human epicardial adipocyte-derived adipokines in the regulation of the myocardial redox state and POAF.
Methods: The authors prospectively studied 274 patients who underwent scheduled open-heart surgery. Finally, 149 patients without known atrial fibrillation were enrolled and divided into POAF and non-POAF groups. Epicardial adipose tissue biopsies and isolated preadipocytes were used to explore the important secretable adipokines associated with POAF.
Results: POAF occurred in 53 (35.6%) patients. Genome-wide expression profiling of background-matched differentiated preadipocytes representing the POAF or non-POAF groups and quantitative polymerase chain reaction validation as a biological replicate using all cohort samples revealed that only SPARCL1 was significantly downregulated in the POAF group. SPARCL1 messenger RNA expression in differentiated preadipocytes correlated with SPARCL1 protein concentration in the preadipocyte culture medium. SPARCL1 attenuated angiotensin II-induced oxidative stress in neonatal rat myocytes. Coculture of human induced pluripotent stem cell-derived atrial cardiomyocyte with the differentiated preadipocyte revealed that the preadipocytes derived from non-POAF patients suppressed myocardial oxidative stress. Low SPARCL1 expression in human epicardial differentiated preadipocytes was associated with a higher risk of long-term adverse cardiovascular events after surgery.
Conclusions: These findings suggested that the SPARCL1, derived from epicardial differentiated preadipocytes, may be an important paracrine adipokine that regulates POAF and subsequent cardiovascular outcomes by improving myocardial redox state.
期刊介绍：
JACC: Clinical Electrophysiology is one of a family of specialist journals launched by the renowned Journal of the American College of Cardiology (JACC). It encompasses all aspects of the epidemiology, pathogenesis, diagnosis and treatment of cardiac arrhythmias. Submissions of original research and state-of-the-art reviews from cardiology, cardiovascular surgery, neurology, outcomes research, and related fields are encouraged. Experimental and preclinical work that directly relates to diagnostic or therapeutic interventions are also encouraged. In general, case reports will not be considered for publication.
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