Treatment response evaluation in an ex vivo model of E. coli-infected central venous catheter system.

IF 2.1 3区 医学 Q2 PEDIATRICS
Frontiers in Pediatrics Pub Date : 2025-06-18 eCollection Date: 2025-01-01 DOI:10.3389/fped.2025.1421992
Zihe Huo, Corinne Légeret, Stefan G Holland-Cunz, Stephanie J Gros
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引用次数: 0

Abstract

Introduction: Despite all precautions, central line-associated bloodstream infections (CLABSI) are inevitable, especially in children. Different treatment strategies exist for those situations. This study aims to compare the different treatment strategies.

Methods: In this study, central venous catheters (CVC, Broviac single lumen) were contaminated with E. coli in vitro. Different treatments (70% ethanol, ceftriaxone, TauroLock) were applied, and the effect was measured by isothermal microcalorimetry.

Results: A rapid decrease in heat release corresponds to a rapid decrease in the number of living bacteria. Ceftriaxone had the quickest effect followed by ethanol in combination with ceftriaxone, ethanol, and TauroLock.

Discussion: Antibiotics must be based on patient risk factors, the severity of infection, and local resistance pattern; therefore, it is difficult to publish general guidelines applying to all children. In this in vitro study, ceftriaxone demonstrated the most the highest efficacy on the bacteria. Taurolidine locks are recommended for preventing CLABSI, but no data are available in regards using it for treatment. In this setting, it was efficient, as was ethanol. However, the bacteria used in this study, have not been exposed to antibiotics before-this is most likely in contrast to patients, who have a central venous catheter.

Conclusion: Under in vitro conditions, systemic ceftriaxone is the most efficient and fastest treatment for an E. coli-infected CVC. Elimination of bacteria was also reached with 70% ethanol and TauroLock, but it needed more time.

大肠杆菌感染中心静脉导管系统的离体模型治疗效果评价。
导语:尽管采取了各种预防措施,但中心线相关血流感染(CLABSI)是不可避免的,特别是在儿童中。针对这些情况存在不同的治疗策略。本研究旨在比较不同的治疗策略。方法:采用体外大肠杆菌污染中心静脉导管(CVC, Broviac单管腔)。采用不同处理(70%乙醇、头孢曲松、TauroLock),采用等温微量热法测定效果。结果:热释放量的迅速减少与活菌数量的迅速减少相对应。头孢曲松效果最快,其次是乙醇与头孢曲松、乙醇和TauroLock联用。讨论:抗生素必须基于患者的危险因素、感染的严重程度和局部耐药模式;因此,很难发布适用于所有儿童的通用指南。在体外实验中,头孢曲松对细菌的治疗效果最好。牛罗列丁锁被推荐用于预防CLABSI,但没有关于使用它治疗的数据。在这种情况下,它和乙醇一样是有效的。然而,在这项研究中使用的细菌之前没有接触过抗生素——这很可能与使用中心静脉导管的患者相反。结论:体外条件下,全身头孢曲松是治疗大肠杆菌感染CVC最有效、最快速的方法。70%乙醇和TauroLock也可以消除细菌,但需要更多的时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Frontiers in Pediatrics
Frontiers in Pediatrics Medicine-Pediatrics, Perinatology and Child Health
CiteScore
3.60
自引率
7.70%
发文量
2132
审稿时长
14 weeks
期刊介绍: Frontiers in Pediatrics (Impact Factor 2.33) publishes rigorously peer-reviewed research broadly across the field, from basic to clinical research that meets ongoing challenges in pediatric patient care and child health. Field Chief Editors Arjan Te Pas at Leiden University and Michael L. Moritz at the Children''s Hospital of Pittsburgh are supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Pediatrics also features Research Topics, Frontiers special theme-focused issues managed by Guest Associate Editors, addressing important areas in pediatrics. In this fashion, Frontiers serves as an outlet to publish the broadest aspects of pediatrics in both basic and clinical research, including high-quality reviews, case reports, editorials and commentaries related to all aspects of pediatrics.
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