The Carba Mix-In Vitro Investigations of Potential Cocktail Effects in Patch Tests With Rubber Allergens.

IF 4.8 1区医学 Q2 ALLERGY

Contact Dermatitis Pub Date : 2025-07-02 DOI:10.1111/cod.14833

Linda Ljungberg Silic, Renato Ivan de Ávila, Sofía Carreira-Santos, Gábor Merényi, Ola Bergendorff, Kathrin S Zeller

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引用次数: 0

Abstract

Background: The carba mix is used to screen rubber chemical allergy and consists of three rubber sensitising chemicals: 1% (w/w) zinc diethyldithiocarbamate (ZDEC), 1% (w/w) zinc dibutyldithiocarbamate (ZDBC) and 1% (w/w) 1,3-diphenylguanidine (DPG), all in petrolatum. However, carba mix patch test results can be difficult to interpret, which may be linked to so-called 'cocktail' effects.

Objectives: The carba mix was used as a 'model mixture' to better understand human reactions observed in clinical patch testing.

Methods: We investigated the responses of a human cell model resembling dendritic cells to the carba mix single constituents ZDEC, ZDBC and DPG and to predefined mixtures capturing the molar composition of the carba mix using transcriptomic approaches and immunological and biochemical assays.

Results: We detected unique cell responses to individual chemicals, where DPG was inducing ROS production and affecting autophagic flux. The combination of ZDEC, ZDBC and DPG induced higher levels of IL-8 secretion than expected based on the IL-8 levels in response to single chemical exposures.

Conclusions: The data collected in this study support the hypothesis that the carba mix can give rise to cocktail effects. Considering exposure profiles, patch testing with separate carba mix sensitisers may be more appropriate.

查看原文本刊更多论文

Carba混合物在橡胶过敏原斑贴试验中潜在鸡尾酒效应的体外研究。

背景：碳水化合物混合物用于筛选橡胶化学过敏，由三种橡胶致敏化学品组成：1% （w/w）二乙基二硫代氨基甲酸锌（ZDEC）， 1% （w/w）二丁基二硫代氨基甲酸锌（ZDBC）和1% (w/w) 1,3-二苯基胍（DPG），均在石油油中。然而，碳水化合物混合斑贴试验的结果很难解释，这可能与所谓的“鸡尾酒”效应有关。目的：carba混合物被用作“模型混合物”，以更好地了解临床贴片试验中观察到的人类反应。方法：我们研究了类似树突状细胞的人类细胞模型对碳水化合物混合物的单一成分ZDEC， ZDBC和DPG的反应，以及使用转录组学方法和免疫学和生化分析捕获碳水化合物混合物的摩尔组成的预定义混合物。结果：我们检测到细胞对单个化学物质的独特反应，其中DPG诱导ROS产生并影响自噬通量。ZDEC、ZDBC和DPG联合使用诱导的IL-8分泌水平高于基于单一化学物质暴露时IL-8水平的预期。结论：本研究收集的数据支持碳水化合物混合物可以产生鸡尾酒效应的假设。考虑到暴露情况，使用单独的碳水化合物混合致敏剂进行斑贴试验可能更合适。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Contact Dermatitis 医学-过敏

CiteScore

4.60

自引率

30.90%

发文量

227

审稿时长

4-8 weeks

期刊介绍： Contact Dermatitis is designed primarily as a journal for clinicians who are interested in various aspects of environmental dermatitis. This includes both allergic and irritant (toxic) types of contact dermatitis, occupational (industrial) dermatitis and consumers" dermatitis from such products as cosmetics and toiletries. The journal aims at promoting and maintaining communication among dermatologists, industrial physicians, allergists and clinical immunologists, as well as chemists and research workers involved in industry and the production of consumer goods. Papers are invited on clinical observations, diagnosis and methods of investigation of patients, therapeutic measures, organisation and legislation relating to the control of occupational and consumers".