{"title":"ZmRLCK1 modulates secondary cell wall deposition in maize","authors":"Haiyan Li, Xin Li, Xiaoduo Lu","doi":"10.1111/tpj.70313","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The secondary cell wall (SCW) is essential for providing mechanical strength to plants, enabling upright growth and facilitating the long-distance transport of water and nutrients through the vascular system. In this study, we generated an EMS-induced mutant library in B73 maize and identified two “drought-sensitive like” mutants, <i>rlck1-1</i> and <i>rlck1-2</i>, which exhibited impaired function of ZmRLCK1. This gene encodes a predicted receptor-like cytoplasmic kinase with kinase activity. Phenotypic analysis revealed that ZmRLCK1 mutation disrupted metaxylem vessel development, likely compromising upward water transport. Additionally, the mutants displayed aberrant microtubule organization, suggesting a role for ZmRLCK1 in cytoskeletal regulation. Differential phosphoproteomic sequencing identified several microtubule-associated proteins as potential substrates of ZmRLCK1, though the exact molecular mechanisms linking ZmRLCK1-mediated phosphorylation to microtubule dynamics remain to be fully elucidated.</p>\n </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"123 1","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.70313","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The secondary cell wall (SCW) is essential for providing mechanical strength to plants, enabling upright growth and facilitating the long-distance transport of water and nutrients through the vascular system. In this study, we generated an EMS-induced mutant library in B73 maize and identified two “drought-sensitive like” mutants, rlck1-1 and rlck1-2, which exhibited impaired function of ZmRLCK1. This gene encodes a predicted receptor-like cytoplasmic kinase with kinase activity. Phenotypic analysis revealed that ZmRLCK1 mutation disrupted metaxylem vessel development, likely compromising upward water transport. Additionally, the mutants displayed aberrant microtubule organization, suggesting a role for ZmRLCK1 in cytoskeletal regulation. Differential phosphoproteomic sequencing identified several microtubule-associated proteins as potential substrates of ZmRLCK1, though the exact molecular mechanisms linking ZmRLCK1-mediated phosphorylation to microtubule dynamics remain to be fully elucidated.
期刊介绍：
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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