Local shear properties of rabbit articular cartilage capture surface region mechanics of human, equine, and bovine tissue

IF 2.4 3区医学 Q3 BIOPHYSICS

Journal of biomechanics Pub Date : 2025-07-02 DOI:10.1016/j.jbiomech.2025.112843

Rebecca M. Irwin , Byumsu Kim , Donghwan Yoon , Daniella M. Gonzalez , Itai Cohen , Lawrence J. Bonassar

{"title":"Local shear properties of rabbit articular cartilage capture surface region mechanics of human, equine, and bovine tissue","authors":"Rebecca M. Irwin , Byumsu Kim , Donghwan Yoon , Daniella M. Gonzalez , Itai Cohen , Lawrence J. Bonassar","doi":"10.1016/j.jbiomech.2025.112843","DOIUrl":null,"url":null,"abstract":"<div><div>New Zealand white rabbits are a prevalent model species used to study preclinical articular cartilage repair therapies. The composition and structure of rabbit articular cartilage have been extensively characterized, yet the local shear properties of the tissue are unknown. Characterizing the local shear properties is essential for understanding the structure–function relationship in the tissue and relating the rabbit preclinical model to human disease. Therefore, the objectives of this study were to (1) characterize the local shear properties of articular cartilage from the femoral condyles of New Zealand white rabbits, (2) determine if local protein content or matrix structure correlated with local shear properties, and (3) compare microscale shear moduli values of rabbit cartilage to those previously reported for human, equine, and bovine tissues. Local shear strains and moduli varied with rabbit cartilage tissue depth; shear modulus was highest ∼ 50 µm below the tissue surface and decreased to plateau values around 150 µm, mirroring the trend with shear strains. Local shear strains showed significant correlations with local protein content but not matrix organization. Rabbit cartilage shear properties followed similar spatial trends as bovine, equine, and human tissue in the first ∼ 100 um of the tissue depth. However, rabbit tissue then differentiated from the larger animals as shear modulus values plateaued and did not increase by an order of magnitude like that seen in the larger species. Local shear properties of rabbit articular cartilage capture the surface properties of human, equine, and bovine cartilage but mechanically lack the deep zone region.</div></div>","PeriodicalId":15168,"journal":{"name":"Journal of biomechanics","volume":"189 ","pages":"Article 112843"},"PeriodicalIF":2.4000,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomechanics","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021929025003550","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

New Zealand white rabbits are a prevalent model species used to study preclinical articular cartilage repair therapies. The composition and structure of rabbit articular cartilage have been extensively characterized, yet the local shear properties of the tissue are unknown. Characterizing the local shear properties is essential for understanding the structure–function relationship in the tissue and relating the rabbit preclinical model to human disease. Therefore, the objectives of this study were to (1) characterize the local shear properties of articular cartilage from the femoral condyles of New Zealand white rabbits, (2) determine if local protein content or matrix structure correlated with local shear properties, and (3) compare microscale shear moduli values of rabbit cartilage to those previously reported for human, equine, and bovine tissues. Local shear strains and moduli varied with rabbit cartilage tissue depth; shear modulus was highest ∼ 50 µm below the tissue surface and decreased to plateau values around 150 µm, mirroring the trend with shear strains. Local shear strains showed significant correlations with local protein content but not matrix organization. Rabbit cartilage shear properties followed similar spatial trends as bovine, equine, and human tissue in the first ∼ 100 um of the tissue depth. However, rabbit tissue then differentiated from the larger animals as shear modulus values plateaued and did not increase by an order of magnitude like that seen in the larger species. Local shear properties of rabbit articular cartilage capture the surface properties of human, equine, and bovine cartilage but mechanically lack the deep zone region.

查看原文本刊更多论文

兔关节软骨的局部剪切特性捕获了人、马和牛组织的表面区域力学

新西兰大白兔是研究临床前关节软骨修复疗法的常用模型物种。兔关节软骨的组成和结构已被广泛表征，但组织的局部剪切特性是未知的。表征局部剪切特性对于理解组织中的结构-功能关系以及将兔临床前模型与人类疾病联系起来至关重要。因此，本研究的目的是：(1)表征新西兰大白兔股骨髁关节软骨的局部剪切特性，(2)确定局部蛋白质含量或基质结构是否与局部剪切特性相关，(3)将兔软骨的微尺度剪切模量值与先前报道的人、马和牛组织的微尺度剪切模量值进行比较。局部剪切应变和模量随兔软骨组织深度的变化而变化；剪切模量在组织表面以下约50µm处最高，并在150µm左右降至平台值，与剪切应变的趋势一致。局部剪切应变与局部蛋白质含量呈显著相关，而与基质组织无显著相关。兔软骨剪切特性在组织深度的第一个~ 100微米范围内与牛、马和人组织具有相似的空间趋势。然而，随着剪切模量趋于稳定，兔的组织与大型动物的组织分化，并没有像大型动物那样增加一个数量级。兔关节软骨的局部剪切特性捕获了人、马和牛软骨的表面特性，但机械上缺乏深层区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of biomechanics 生物-工程：生物医学

CiteScore

5.10

自引率

4.20%

发文量

345

审稿时长

1 months

期刊介绍： The Journal of Biomechanics publishes reports of original and substantial findings using the principles of mechanics to explore biological problems. Analytical, as well as experimental papers may be submitted, and the journal accepts original articles, surveys and perspective articles (usually by Editorial invitation only), book reviews and letters to the Editor. The criteria for acceptance of manuscripts include excellence, novelty, significance, clarity, conciseness and interest to the readership. Papers published in the journal may cover a wide range of topics in biomechanics, including, but not limited to: -Fundamental Topics - Biomechanics of the musculoskeletal, cardiovascular, and respiratory systems, mechanics of hard and soft tissues, biofluid mechanics, mechanics of prostheses and implant-tissue interfaces, mechanics of cells. -Cardiovascular and Respiratory Biomechanics - Mechanics of blood-flow, air-flow, mechanics of the soft tissues, flow-tissue or flow-prosthesis interactions. -Cell Biomechanics - Biomechanic analyses of cells, membranes and sub-cellular structures; the relationship of the mechanical environment to cell and tissue response. -Dental Biomechanics - Design and analysis of dental tissues and prostheses, mechanics of chewing. -Functional Tissue Engineering - The role of biomechanical factors in engineered tissue replacements and regenerative medicine. -Injury Biomechanics - Mechanics of impact and trauma, dynamics of man-machine interaction. -Molecular Biomechanics - Mechanical analyses of biomolecules. -Orthopedic Biomechanics - Mechanics of fracture and fracture fixation, mechanics of implants and implant fixation, mechanics of bones and joints, wear of natural and artificial joints. -Rehabilitation Biomechanics - Analyses of gait, mechanics of prosthetics and orthotics. -Sports Biomechanics - Mechanical analyses of sports performance.