Performance evaluation of antimicrobial blue light for inactivating multidrug-resistant bacteria on common fabric materials

IF 4 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-05-28 DOI:10.1016/j.jiph.2025.102846

Hanlin Liu , Huihui Zhang , Alvin C.K. Lai

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引用次数: 0

Abstract

Background

Fomite transmission of multidrug-resistant bacteria in hospitals is a serious public health issue. Antimicrobial blue light (aBL) holds great promise in reducing the incidence of healthcare-associated infections (HAIs) while being safe for use.

Methods

This study evaluated the influence of surface materials on the bactericidal performance of aBL against three multidrug-resistant bacteria: Methicillin-resistant Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Six microbial-contaminated surfaces were studied: glass, cotton, towel, blanket, sofa and bedsheet.

Results

At the same dose of 3.3 J/cm², aBL treatment for bedsheets resulted in a 3.0 times higher log reduction in colony forming counts (CFUs) than sofa material. This significant difference may be partly due to fabric porosity and variations in light penetration. Additionally, a positive linear relationship between reactive oxygen species (ROS) generation and microbial reduction was established, indicating that higher intracellular ROS levels corresponded linearly to more effective bactericidal performance by aBL. We also systematically compared the penetration and bactericidal performance of aBL with 254 nm Ultraviolet C (UVC) and 222 nm Far-UVC for testing double-layer cotton samples. Both UVC and Far-UVC outperformed aBL treatment for single-layer fabrics. Nonetheless, due to the very low light penetration of shorter wavelengths, the bactericidal efficiency of UVC and Far-UVC was substantially reduced for the bottom-layer sample. For aBL, the difference in bactericidal efficiency data between top-layer and bottom-layer was approximately 10 %.

Conclusion

aBL exhibited varying bactericidal efficacy across different surfaces, with the highest microbial reduction observed on bedsheet and the lowest reduction on sofa fabric. Furthermore, aBL demonstrated superior bactericidal performance against drug-resistant bacteria on multi-layer fabrics compared to Far-UVC and UVC.

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抗菌蓝光灭活常见织物材料多重耐药菌的性能评价

背景：多药耐药细菌在医院的传播是一个严重的公共卫生问题。抗菌蓝光（aBL）在安全使用的同时，在减少卫生保健相关感染（HAIs）的发生率方面具有很大的希望。方法研究表面材料对aBL对耐甲氧西林金黄色葡萄球菌、肺炎克雷伯菌和铜绿假单胞菌3种多重耐药菌杀菌性能的影响。研究了六种被微生物污染的表面：玻璃、棉花、毛巾、毯子、沙发和床单。结果在相同剂量（3.3 J/cm2）下，aBL处理床单的菌落形成计数（CFUs）对数降幅比沙发材料高3.0倍。这种显著的差异可能部分是由于织物孔隙度和光线穿透的变化。此外，活性氧（ROS）的产生与微生物的减少之间存在正线性关系，表明细胞内ROS水平越高，aBL的杀菌效果越好。我们还系统地比较了aBL对254 nm紫外C （UVC）和222 nm远紫外C （Far-UVC）对双层棉样品的渗透和杀菌性能。UVC和Far-UVC处理单层织物的效果都优于aBL处理。然而，由于短波长的光穿透率非常低，底层样品的UVC和Far-UVC的杀菌效率大大降低。对于aBL，顶层与底层的杀菌效率数据差异约为10 %。结论abl在不同表面的杀菌效果不同，床单上的微生物减少率最高，沙发织物上的微生物减少率最低。此外，aBL在多层织物上对耐药细菌的杀菌性能优于Far-UVC和UVC。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.