Microneedle Sensors for Ion Monitoring in Plants. One Step Closer to Smart Agriculture.

Abstract

As global demand for food rises and agricultural systems face unprecedented stress from environmental challenges, understanding the role of ions (i.e., key nutrient components) in crop productivity has never been more critical. Unfortunately, current tools for ion analysis in plants rely on destructive sap collection that fails to capture the dynamic changes in ionic concentrations. On the other hand, noninvasive optical methods lack practicality for field applications due to their reliance on expensive equipment and complex operational procedures. Recent advancements in microneedle (MN) sensing technology have demonstrated significant potential for real-time monitoring of plants' health by enabling the direct detection of various important biomarkers, including but not limited to ions. By offering a minimally invasive approach, MN sensors allow continuous in-planta monitoring with precise penetration into plant tissues, ensuring natural growth remains undisturbed. However, the application of MN sensors, especially for in vivo ion measurement, is still in its very early stage. Herein, we delve into the technological potential and application avenues of plant MN sensors, with a focus on tailoring sensor designs to meet the specific requirements of various plant growth environments and analytical performances for ion detection. This perspective paper also introduces the essential relevance of ion levels in plants, provides a comprehensive assessment of existing ion detection methods, and identifies key challenges associated with achieving effective in planta monitoring. Notably, we highlight the potential of MN sensors as a transformative approach for unveiling plant stress responses, optimizing crop yields, and fulfilling diverse roles that bridge the fields of precision agriculture and plant science research.