Cu2+-activatable fluorescence and chemiluminescence dual-mode sensing platform for visual detection of thiram

Abstract

Herein, we developed a Cu²⁺-activatable dual-mode fluorescence and chemiluminescence probe (Ac-Do-Py) for thiram detection via competitive binding, achieving a detection limit of 100 nM under chemiluminescence mode. This first portable platform with dual fluorescence/chemiluminescence responses enables in situ monitoring of thiram residues on fruit surfaces.