The N gene protects tomato plants from tomato brown rugose fruit virus infection

IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Journal Pub Date : 2025-07-02 DOI:10.1111/pbi.70237

Jing Zhou, Andrea Gilliard, Jeffrey Tung, Savithramma P. Dinesh‐Kumar, Steven A. Whitham, Barbara Baker, Kai‐Shu Ling

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引用次数: 0

Abstract

SummaryThe tobamovirus tomato brown rugose fruit virus (ToBRFV) has recently emerged, causing significant damage to the tomato industry in various regions worldwide, including the US. ToBRFV evades the widely used Tm‐22 resistance gene, which encodes a nucleotide‐binding leucine‐rich repeat (NLR) class immune receptor with an N‐terminal coiled‐coil (CC) domain that confers resistance to the tomato mosaic virus (ToMV). In this study, we tested a transgenic tomato line (tomatoNN) expressing the Nicotiana glutinosa N gene, which encodes an NLR with a Toll‐Interleukin 1 homology domain (TIR) at the N‐terminus, for resistance to ToBRFV. Our results demonstrate that tomatoNN is resistant to ToBRFV, evidenced by the necrotic local lesions observed on the inoculated leaves and the absence of symptoms on systemic leaves. This correlates with very low to non‐detectable virus levels in double antibody sandwich enzyme‐linked immunosorbent (DAS‐ELISA) and quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR) assays. Furthermore, our findings reveal that tomatoNN is resistant to ToBRFV at 22 °C, but not at 30 °C, showing that the temperature‐sensitive nature of N‐mediated resistance also extends to ToBRFV resistance in tomato. These results highlight the significant potential of using tomatoNN to breed tomato cultivars resistant to ToBRFV, offering a new approach to managing the global pandemic caused by this emerging virus.

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N基因保护番茄植株免受番茄褐皱果病毒的感染

摘要最近出现的托巴莫病毒番茄褐皱果病毒（ToBRFV）对包括美国在内的世界各地的番茄产业造成了严重的危害。ToBRFV避开了广泛使用的Tm - 22抗性基因，该基因编码一个核苷酸结合的富含亮氨酸的重复序列（NLR）类免疫受体，该受体具有N -末端卷曲（CC）结构域，赋予对番茄花叶病毒（ToMV）的抗性。在这项研究中，我们测试了一个表达Nicotiana glutinosa N基因的转基因番茄株系（tomatoNN），该基因编码在N端具有Toll‐白细胞介素1同源结构域（TIR）的NLR，以抵抗ToBRFV。我们的研究结果表明，番茄n对ToBRFV具有抗性，这可以从接种叶片上观察到的局部坏死病变和全身叶片上没有症状来证明。这与双抗体夹心酶联免疫吸附（DAS - ELISA）和定量逆转录-聚合酶链反应（RT - qPCR）检测中非常低至不可检测的病毒水平相关。此外，我们的研究结果表明，番茄N在22°C时对ToBRFV具有抗性，而在30°C时则没有，这表明N介导的抗性对温度敏感的性质也延伸到番茄的ToBRFV抗性。这些结果突出了利用番茄n培育抗ToBRFV番茄品种的巨大潜力，为管理这种新出现的病毒引起的全球大流行提供了一种新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Biotechnology Journal 生物-生物工程与应用微生物

CiteScore

20.50

自引率

2.90%

发文量

201

审稿时长

1 months

期刊介绍： Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.