Water-insensitive NIR-I-to-NIR-I down-shifting nanoparticles enable stable biomarker detection at low power thresholds in opaque aqueous environments

IF 20.6 Q1 OPTICS
Dongkyu Kang, Suyeon Kim, Yeongchang Goh, Minseo Kim, Sun-Hak Lee, Jung-Hoon Kwon, Sang Hwan Nam, Joonseok Lee
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Abstract

Luminescence quenching in aqueous environments poses a challenge for practical applications. Lanthanide-doped up-conversion nanoparticles (UCNPs), representative of near-infrared (NIR)-emitting phosphors, typically utilize Yb3+ ions as sensitizers, requiring 980 nm light. This wavelength coincides with the transitions of water molecules, interfering with population dynamics, and continuous irradiation causes unintended heating. Although Nd3+ ions, which absorb at 800 nm, serve as alternative sensitizers, their practical use is limited by low quantum yield (Q.Y.). In this study, we developed water-insensitive down-shifting nanoparticles (WINPs) functioning within the NIR-I range (700–900 nm) to avoid water interference. Characterization through single-particle-level spectroscopy demonstrated water-insensitive properties, with identical powers density and lifetime profiles under both dry and water conditions. The WINPs achieved a high Q.Y. of 22.1 ± 0.9%, allowing operation at a detection limit power 15-fold lower than UCNPs, effectively eliminating background noise and enhancing overall performance. To assess diagnostic potential, we validated WINP-based lateral flow immunoassay (LFA) for detecting avian influenza viruses (AIVs) in 65 opaque clinical samples, achieving 100% sensitivity and an area under the curve (AUC) of 1.000 at only 100 mW cm−2. These findings highlight the potential of WINPs as water-insensitive NIR phosphors that can operate at low power, even in water-rich environments.

Abstract Image

对水不敏感的NIR-I-to-NIR-I下移纳米颗粒能够在不透明的水环境中以低功率阈值稳定地检测生物标志物
水环境下的发光猝灭对实际应用提出了挑战。镧系掺杂上转换纳米粒子(UCNPs)是近红外(NIR)发光荧光粉的代表,通常使用Yb3+离子作为敏化剂,需要980 nm的光。这个波长与水分子的跃迁相吻合,干扰了种群动态,并且持续的辐射会导致意外的加热。虽然在800 nm吸收的Nd3+离子可以作为替代敏化剂,但其实际应用受到低量子产率(q.y)的限制。在这项研究中,我们开发了在NIR-I范围(700-900 nm)内运行的水不敏感下移纳米颗粒(WINPs),以避免水干扰。通过单粒子级光谱表征,证明了其对水不敏感的特性,在干燥和水条件下具有相同的功率密度和寿命曲线。WINPs实现了22.1±0.9%的高q.y,允许在比UCNPs低15倍的检测极限功率下运行,有效地消除了背景噪声并提高了整体性能。为了评估诊断潜力,我们验证了基于winp的侧流免疫分析法(LFA)在65份不透明临床样本中检测禽流感病毒(AIVs),在仅100 mW cm - 2的条件下实现了100%的灵敏度和1.000的曲线下面积(AUC)。这些发现强调了WINPs作为水不敏感的近红外荧光粉的潜力,即使在富含水的环境中,也可以在低功率下工作。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Light-Science & Applications
Light-Science & Applications 数理科学, 物理学I, 光学, 凝聚态物性 II :电子结构、电学、磁学和光学性质, 无机非金属材料, 无机非金属类光电信息与功能材料, 工程与材料, 信息科学, 光学和光电子学, 光学和光电子材料, 非线性光学与量子光学
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