The length and strength of compartmental interactions are modulated by condensin II activity.

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
Randi Isenhart, Son C Nguyen, Leah Rosin, Weihuan Cao, Patrick Walsh, Haris Muzaffar, Christopher E Ellison, Eric F Joyce
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引用次数: 0

Abstract

The spatial organization of the genome is crucial for its function and integrity. Although the ring-like SMC complex condensin II has a well-documented role in organizing mitotic chromosomes, its function in interphase chromatin structure has remained more enigmatic. Using a combination of Oligopaint fluorescence in situ hybridization (FISH) and Hi-C, we show that altering condensin II levels in diploid Drosophila cells significantly changes chromosome architecture at large length scales between chromatin compartments. Notably, condensin II overexpression disrupts the robust boundary between heterochromatin and euchromatin, leading to interactions that span entire chromosomes. These interactions occur independent from transcriptional changes, suggesting that the mechanisms driving compartment formation and their interactions might be distinct aspects of genome organization. Our results provide new insights into the dynamic nature of chromosome organization and underscore the importance of condensin II in maintaining genomic stability.

室区相互作用的长度和强度由凝缩蛋白II活性调节。
基因组的空间组织对其功能和完整性至关重要。尽管环状SMC复合体凝缩蛋白II在有丝分裂染色体的组织中发挥了充分的作用,但其在间期染色质结构中的功能仍然是一个谜。利用寡聚颜料荧光原位杂交(FISH)和Hi-C的结合,我们发现改变二倍体果蝇细胞中凝聚素II的水平显著改变了染色质室之间大长度尺度的染色体结构。值得注意的是,凝缩蛋白II过表达破坏了异染色质和常染色质之间的强大边界,导致跨越整个染色体的相互作用。这些相互作用独立于转录变化,表明驱动室形成的机制及其相互作用可能是基因组组织的不同方面。我们的研究结果为染色体组织的动态性质提供了新的见解,并强调了凝聚蛋白II在维持基因组稳定性中的重要性。
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来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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