Quantitative phase imaging in Hadamard-based active single-pixel microscopy by the motionless transport of intensity equation.

IF 3.1 2区 物理与天体物理 Q2 OPTICS
Optics letters Pub Date : 2025-07-01 DOI:10.1364/OL.566044
Samuel I Zapata-Valencia, Heberley Tobon-Maya, Osamu Matoba, Jesús Lancis, Enrique Tajahuerce
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引用次数: 0

Abstract

In this Letter, a method to achieve quantitative phase imaging (QPI) on Hadamard-based single-pixel microscopy (HSPM) is presented. A motionless implementation of the transport of intensity equation (TIE) supported by a focus-tunable lens (FTL) is reported for HSPM. The presented approach enables the use of digital micromirror devices (DMDs) instead of the typically implemented phase-only modulators utilized in QPI single-pixel imaging (SPI). Photobleaching and phototoxicity are minimized under the proposed approach. A minimal light-sample interaction is guaranteed by the DMDs' achievable frame rates, the structured illumination approach, and the use of high-sensitive photodiodes. The feasibility of this method is validated by imaging a calibrated phase-only USAF test target of different height steps. Additionally, epithelial cheek cells are imaged under low-power conditions, 240 µW, to validate the effectiveness of phase retrieval for biological samples.

基于hadamard的有源单像素显微镜中强度方程的静止输运定量相位成像。
本文提出了一种在基于hadamard的单像素显微镜(HSPM)上实现定量相位成像(QPI)的方法。本文报道了一种由可调焦透镜(FTL)支持的强度传递方程(TIE)的静止实现。所提出的方法能够使用数字微镜器件(dmd)而不是QPI单像素成像(SPI)中使用的典型实现的纯相位调制器。该方法最大限度地减少了光漂白和光毒性。最小的光样品相互作用是由dmd可实现的帧速率,结构化照明方法和高灵敏度光电二极管的使用保证的。通过对不同高度步长校准后的纯相位USAF测试目标进行成像,验证了该方法的可行性。此外,在低功率条件下(240 μ W)对脸颊上皮细胞进行成像,以验证生物样品相检索的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Optics letters
Optics letters 物理-光学
CiteScore
6.60
自引率
8.30%
发文量
2275
审稿时长
1.7 months
期刊介绍: The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.
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