Modulating transformation of DNA origami nanoarray via sequence design.

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-07-01 DOI:10.1038/s41467-025-61421-w

Dongfang Wang, Fiona Cole, Martina Pfeiffer, Mengting Cao, Tim Schröder, Philip Tinnefeld, Yonggang Ke

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Abstract

The four-way DNA junction is the most prevalent structural motif in DNA nanotechnology. Recently, a reconfigurable DNA nanoarray (domino array) was created with this basic motif to realize intricate, stepwise transformation by the information relay between neighboring four-way junction units. Here, we generate a DNA domino array with same sequences at every junction, and use it as a platform to study how the design of DNA bases at junctions influences the kinetics and thermodynamics of transformation of four-way junctions in reconfigurable DNA nanoarrays. By regulating the energy difference and thus the conversion between the two configurations of four-way junctions, we show the transformation of DNA nanoarray can be modulated in a designable manner. The coordinated transformation of four-way junctions in the DNA domino array enables a detailed investigation on array transformation by using Atomic Force Microscopy (AFM) imaging and single-molecule Förster resonance energy transfer (FRET) microscopy.

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基于序列设计的DNA折纸纳米阵列调制转化。

四向DNA连接是DNA纳米技术中最普遍的结构基序。近年来，利用这一基本基序构建了一种可重构的DNA纳米阵列（骨牌阵列），通过相邻四向结单元之间的信息中继实现复杂的逐步转换。在这里，我们生成了一个在每个结点上具有相同序列的DNA多米诺骨牌阵列，并以此为平台研究了在可重构DNA纳米阵列中，结点上DNA碱基的设计如何影响四向结点转化的动力学和热力学。通过调节能量差，从而在四向结的两种配置之间的转换，我们表明DNA纳米阵列的转换可以以可设计的方式进行调制。利用原子力显微镜（AFM）成像和单分子Förster共振能量转移显微镜（FRET）对DNA骨牌阵列中四向结的协同转化进行了详细的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.