Characterization, antioxidant activity of banana peel carbon dots and their antibacterial mechanism against Staphylococcus aureus.

IF 3.2 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Kai Fan, Rui Tang, Lijing Li
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引用次数: 0

Abstract

Aims: The carbon dots (CDs) used in this study are a type of carbon nanomaterial extracted from banana peels, which possess various biological activities and demonstrate significant advantages in the antimicrobial field. Staphylococcus aureus (S. aureus) is one of the most common foodborne pathogenic bacteria, severely impacting human health and food safety, as well as causing substantial economic losses. This study investigates the properties of the CDs and their inhibition mechanism against S. aureus.

Methods and results: The synthesized CDs were characterized using transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV-Vis), fluorescence spectroscopy, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The antioxidant activity and antimicrobial effect of CDs were explored through free radical scavenging and bacterial experiments. The cytocompatibility of CDs was determined by MTT assay. The results showed that spherical blue CDs of 6.4 ± 1.56 nm were synthesized. CDs showed a UV-visible absorption peak at 265 nm. CDs are rich in hydroxyl, carboxyl, and amino groups, demonstrating excellent water solubility, photostability, pH stability, and antioxidant properties. The findings showed that CDs disrupt the cell membrane structure of S. aureus, resulting in altered membrane permeability, leakage of cell contents, and accumulation of excessive ROS. The minimum inhibitory concentration (MIC) was found to be 3.125 mg mL-1. Moreover, the CDs exhibited good cell compatibility.

Conclusion: CDs are an ideal antioxidant and natural antibacterial agent that can inhibit S. aureus. The antioxidant effect of CDs is achieved through radical scavenging, and they exhibit good cytocompatibility. At the same time, CDs exhibits strong antifungal activity through mechanisms such as disrupting the cell membrane structure, altering membrane permeability, causing leakage of cellular contents, increasing MDA levels, and accumulating intracellular ROS.

香蕉皮碳点的表征、抗氧化活性及其对金黄色葡萄球菌的抑菌机制。
目的:本研究使用的碳点(cd)是从香蕉皮中提取的一种碳纳米材料,具有多种生物活性,在抗菌领域具有显著优势。金黄色葡萄球菌(S. aureus)是最常见的食源性致病菌之一,严重影响人类健康和食品安全,并造成重大经济损失。本研究探讨了CDs的性质及其对金黄色葡萄球菌的抑制机制。方法与结果:采用透射电子显微镜(TEM)、紫外可见光谱(UV-Vis)、荧光光谱、x射线衍射(XRD)和傅里叶变换红外光谱(FTIR)对合成的CDs进行了表征。通过自由基清除和细菌实验,探讨了CDs的抗氧化活性和抗菌作用。采用MTT法测定CDs的细胞相容性。结果表明,合成了尺寸为6.4±1.56 nm的球形蓝色CDs。CDs在265 nm处有紫外可见吸收峰。CDs富含羟基、羧基和氨基,具有良好的水溶性、光稳定性、pH稳定性和抗氧化性能。研究结果表明,CDs破坏了金黄色葡萄球菌的细胞膜结构,导致膜通透性改变,细胞内容物渗漏,过量ROS积累。最小抑菌浓度(MIC)为3.125 mg mL-1。此外,cd表现出良好的细胞相容性。结论:CDs是一种理想的抗氧化剂和天然抗菌药物,具有抑制金黄色葡萄球菌的作用。CDs的抗氧化作用是通过清除自由基来实现的,它们具有良好的细胞相容性。同时,CDs通过破坏细胞膜结构、改变细胞膜通透性、引起细胞内容物渗漏、增加MDA水平、积累细胞内ROS等机制表现出较强的抗真菌活性。
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来源期刊
Journal of Applied Microbiology
Journal of Applied Microbiology 生物-生物工程与应用微生物
CiteScore
7.30
自引率
2.50%
发文量
427
审稿时长
2.7 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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