Capillary Electrophoresis Can Detect the Simultaneous Presence of Hairpins and Self-Dimers in Non-Symmetric, Single-Stranded DNA Oligomers.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-07-02 DOI:10.1002/elps.70005

Earle Stellwagen, Nancy C Stellwagen

引用次数: 0

Abstract

Free solution capillary electrophoresis (CE) has been used to show that non-symmetric, single-stranded DNA oligomers containing 26 nucleotides can exhibit peaks in the electropherograms that correspond to the simultaneous presence of self-dimers and hairpins in the solution. The overlapping hairpin and self-dimer peaks were observed at temperatures close to 15°C in background electrolytes containing at least 80 mM Na⁺ ions. With increasing temperature, the self-dimers were converted first into hairpins and then into random coils at still higher temperatures. The results suggest that hairpins can be an intermediary step in the pathway between DNA duplexes and single-strands.

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毛细管电泳可以检测非对称单链DNA低聚物中同时存在的发夹和自二聚体。

自由溶液毛细管电泳（CE）显示，含有26个核苷酸的非对称单链DNA低聚物在电泳图中显示出峰，对应于溶液中同时存在自二聚体和发夹。在温度接近15°C时，在含有至少80 mM Na+离子的背景电解质中观察到重叠的发夹峰和自二聚体峰。随着温度的升高，自二聚体首先转变为发夹，然后在更高的温度下转变为随机线圈。结果表明，发夹可能是DNA双链和单链之间途径的中间步骤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.