Reference values for anti-Müllerian hormone measured by electrochemiluminescence in a pediatric population in Argentina.

Abstract

Introduction. Anti-Müllerian hormone (AMH) is a specific marker of gonadal Sertoli cells, usually used for the diagnosis of disorders of sexual development. Currently, automated methods for measuring AMH are routinely incorporated into clinical laboratories. Objective. To obtain reference values of AMH in a population of healthy neonates and infants using an electrochemiluminescent immunoassay (ECLIA). Population and methods. We analyzed 599 serum samples from healthy neonates and infants (F = 296, M = 303) aged 2 to 291 days, with a distribution as follows: ≤15 days (n = 352) and >15 days (n = 247). An ECLIA-Roche™ assay determined AMH. AMH distribution percentiles were calculated using the Bootstrap method. Results. AMH values (ng/mL) in neonates and infants were: M: (≤15 days: P2.5: 27.5; P97.5: 118.3; >15 days: P2.5: 13.9; P97.5: 186.0. F: ≤15 days: P2.5: 0.03; P97.5: 4.00; >15 days: P2.5: 0.03; P97.5: 11.5. Conclusion. Our work provides reference values of AMH according to age and sex, facilitating its appropriate clinical use in the endocrinological evaluation of a population. The results obtained are consistent with previous studies, which show that AMH values are elevated at birth in males, reflecting the expression of Sertoli cell functionality.