Shuichiro Suzuki, Ken Imajo, Junfeng Wang, Jeonghyun Kim, Eijiro Maeda, Kazuaki Nagayama, Takeo Matsumoto
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引用次数: 0
Abstract
When cyclic stretch is applied to a monolayer of cells cultured on an elastic substrate, many types of cells align in the direction perpendicular to the stretch or along the direction of minimal substrate strain. However, the behavior of multilayer cells under cyclic stretch remains unclear. In this study, we cultured MC3T3-E1 osteoblast-like cells at high density to form multilayer cells and subjected them to cyclic stretch with an amplitude of 10% at 1 Hz. We found that the lower layer cells aligned in the direction of the stretch after 12 h, whereas the upper layer cells aligned perpendicular to the direction of stretch after 24 h. The 10% cyclic stretch was transmitted to the upper layer cells as approximately 5% at the onset of the stretch and increased over time, reaching 7% at 12 h when the lower layer cells completed alignment in the direction of stretch. This suggests that sufficient cyclic stretch transmitted to the upper layer led to the alignment of the upper layer cells in the perpendicular direction after 12 h. On the other hand, reducing intracellular tension with Y-27632 caused cells in both upper and lower layers to align in the direction of stretch. In contrast, increasing intracellular tension with calyculin A eliminated significant alignment in both layers. These findings indicate that cell alignment is closely related to intracellular tension and that the alignment of the lower layer cells in the direction of stretch may be due to a decrease in intracellular tension.
期刊介绍:
Mechanics regulates biological processes at the molecular, cellular, tissue, organ, and organism levels. A goal of this journal is to promote basic and applied research that integrates the expanding knowledge-bases in the allied fields of biomechanics and mechanobiology. Approaches may be experimental, theoretical, or computational; they may address phenomena at the nano, micro, or macrolevels. Of particular interest are investigations that
(1) quantify the mechanical environment in which cells and matrix function in health, disease, or injury,
(2) identify and quantify mechanosensitive responses and their mechanisms,
(3) detail inter-relations between mechanics and biological processes such as growth, remodeling, adaptation, and repair, and
(4) report discoveries that advance therapeutic and diagnostic procedures.
Especially encouraged are analytical and computational models based on solid mechanics, fluid mechanics, or thermomechanics, and their interactions; also encouraged are reports of new experimental methods that expand measurement capabilities and new mathematical methods that facilitate analysis.