Jiao San Xian granule containing prebiotic alleviate functional dyspepsia by regulating intestinal flora and the secretion of inflammatory response.

IF 2.6 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Huimin Pei, Mengyuan Zhao, Hongyue Wang, Xindi Zhang, Caihong Shi, Xiangrong Zhang
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Abstract

Jiao Sanxian (JSX) consists of three traditional Chinese medicine including charred hawthorn, malt and Liu Shen Qu, they are also from Chinese medicine homologous food. It is commonly used to relieve functional dyspepsia (FD). The present study aims to explore the mechanism of JSX (granule), with the addition of prebiotics for the treatment of FD using mice model. The network pharmacology and molecular docking were used to forecast therapeutic targets for JSX. Based on the protein-protein interaction (PPI) results, thirteen targets exhibited strong interactions with each other. The results of network pharmacology and molecular docking revealed therapeutic activity of JSX in FD regulated by its anti-inflammatory effect, improvement in gastrointestinal function and physiological activity in components-targets-pathways-disease. The pharmacological effects of JSX were further verified by rRNA gene sequencing, enzyme-linked immunosorbent assay (ELISA) and animal study. The in vivo results showed JSX, in combination with prebiotics, could mitigate FD symptoms. ELISA study showed that glyceraldehyde-3-phosphate dehydrogenase (GAPDH), AKT1 and tumor necrosis factor (TNF)-α played the roles in reducing the inflammatory response by downregulating inflammation content. The above results were coincided with the network pharmacology prediction. By 16S rRNA, the results showed JSX may alleviate the FD symptom by increasing the diversity of the intestinal flora, adjusting the dominant prebiotic. In conclusion, JSX can alleviate functional dyspepsia in mice. The mechanisms were related to regulating the secretion of inflammatory response, significantly improving the benefits of intestinal flora and ameliorating multi-metabolic pathways.

含益生元的焦三仙颗粒通过调节肠道菌群和炎症反应分泌来缓解功能性消化不良。
焦三鲜(JSX)由焦山楂、麦芽、六神曲三种中药组成,也是中药同源食品。常用于缓解功能性消化不良(FD)。本研究旨在通过小鼠模型探讨JSX(颗粒)在添加益生元的情况下治疗FD的作用机制。利用网络药理学和分子对接技术预测JSX的治疗靶点。根据蛋白-蛋白相互作用(PPI)结果,13个靶点表现出强相互作用。网络药理学和分子对接结果显示,JSX对FD的治疗活性受其抗炎作用、胃肠功能改善和组分-靶点-通路-疾病生理活性调控。通过rRNA基因测序、酶联免疫吸附试验(ELISA)和动物实验进一步验证JSX的药理作用。体内实验结果显示,JSX联合益生元可减轻FD症状。ELISA研究表明,甘油醛-3-磷酸脱氢酶(GAPDH)、AKT1和肿瘤坏死因子(TNF)-α通过下调炎症含量来减轻炎症反应。上述结果与网络药理学预测相吻合。通过16S rRNA,结果显示JSX可能通过增加肠道菌群多样性,调节优势益生元来缓解FD症状。综上所述,JSX可减轻小鼠功能性消化不良。其机制可能与调节炎症反应的分泌,显著改善肠道菌群的益处,改善多种代谢途径有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Food and Drug Analysis
Journal of Food and Drug Analysis 医学-食品科技
CiteScore
6.30
自引率
2.80%
发文量
36
审稿时长
3.8 months
期刊介绍: The journal aims to provide an international platform for scientists, researchers and academicians to promote, share and discuss new findings, current issues, and developments in the different areas of food and drug analysis. The scope of the Journal includes analytical methodologies and biological activities in relation to food, drugs, cosmetics and traditional Chinese medicine, as well as related disciplines of topical interest to public health professionals.
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