Optical tweeze-sectioning microscopy for 3D imaging and manipulation of suspended cells

IF 12.5 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-07-02 DOI:10.1126/sciadv.adx3900

Xing Li, Dan Dan, Siarhei Zavatski, Wenyu Gao, Qiang Zhang, Yuan Zhou, Jia Qian, Yanlong Yang, Xianghua Yu, Shaohui Yan, Xiaohao Xu, Olivier J. F. Martin, Baoli Yao

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Abstract

Optical manipulation and detection of biological particulates are crucial procedures in biophotonics. Optical sectioning (OS) opens the avenue to three-dimensional (3D) microscopy, but nonoptical approaches, including sample adhesion and mechanical scanning, have always been required in this technique, rendering it impossible to image suspended cells. Here, we develop optical tweeze-sectioning microscopy by coupling structured illumination microscopy (SIM) with holographic optical tweezers (HOTs). By sculpting light in HOTs, we demonstrate that the position fluctuations of suspended yeast cells can be optically squeezed to tens of nanometers, which is sufficient for the implementation of OS with SIM. Sample scanning is achieved through optical delivery of the cells, instead of translation stages as in the conventional way. Our work presents an all-optical solution for OS, broadening its application to nonadherent, suspended cells. It further furnishes the original technique that enables both SIM-based 3D imaging and optical manipulation.

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用于悬浮细胞三维成像和操作的光学镊子切片显微镜

光学操作和生物粒子的检测是生物光子学的关键步骤。光学切片（OS）为三维（3D）显微镜开辟了道路，但该技术一直需要非光学方法，包括样品粘附和机械扫描，这使得不可能对悬浮细胞成像。在这里，我们将结构照明显微镜（SIM）与全息光学镊子（HOTs）耦合在一起，开发了光学镊子切片显微镜。通过在HOTs中雕刻光，我们证明悬浮酵母细胞的位置波动可以被光学压缩到几十纳米，这足以实现具有SIM的OS。样品扫描是通过细胞的光学传递来实现的，而不是像传统方式那样的翻译阶段。我们的工作提出了OS的全光解决方案，将其应用范围扩大到非贴壁悬浮细胞。它进一步提供了原始技术，使基于sim的3D成像和光学操作成为可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.