Contribution to an efficient diagnosis of Fasciola hepatica infection in naturally infected cattle

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-06-26 DOI:10.1016/j.vprsr.2025.101301

Natã Medeiros Godinho , Camila Zomer Spíndola , Márcia Sangaletti Lavina , Andreas Lazaros Chryssafidis , Anderson Barbosa de Moura

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引用次数: 0

Abstract

The control of fascioliasis caused by Fasciola hepatica depends on early detection and assessment of infection severity. A definitive diagnosis is the post-mortem recovery of flukes in the hepatic ducts and gallbladder, which is not feasible for herd monitoring. Coproparasitological techniques based on egg detection in feces are commonly used. This study evaluated the accuracy of the Dennis, Stone, and Swanson (DSS), quatro tamises (QT), and Gordon and Whitlock modified (GWM) techniques using parasitized livers as the gold standard. Of 784 carcasses, 105 (13.39 %) were parasitized by F. hepatica. The sensitivity was 43.8 % for DSS, 86.6 % for QT, and 7.6 % for GWM. In comparison with the gold standard, agreement on the Kappa scale was weak for GWM (0.10), moderate for DSS (0.45), and excellent for QT (0.84). Additionally, when comparing the techniques with each other, modest agreement was observed between QT and GWM (0.20), and moderate agreement between DSS and GWM (0.57) and DSS and QT (0.48). Parasite intensity did not influence (P > 0.05) the sensitivity of DSS and QT. These findings indicate that QT is the most sensitive and reliable coproparasitological technique for diagnosing F. hepatica infection in naturally infected cattle.

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对自然感染牛肝片形吸虫感染的有效诊断贡献

肝片形吸虫引起的片形吸虫病的控制取决于早期发现和感染严重程度的评估。一个明确的诊断是死后在肝管和胆囊中发现吸虫，这对于牛群监测是不可行的。基于粪便中卵检测的粪寄生虫学技术是常用的。本研究评估了Dennis， Stone, and Swanson (DSS), quatro tamises （QT）和Gordon and Whitlock modified （GWM）技术的准确性，这些技术以寄生肝脏为金标准。在784具尸体中，有105具（13.39%）被肝单胞菌寄生。DSS的敏感性为43.8%，QT为86.6%，GWM为7.6%。与金标准相比，Kappa量表对GWM的一致性较弱（0.10），对DSS的一致性中等（0.45），对QT的一致性极好（0.84）。此外，在相互比较技术时，QT与GWM（0.20）、DSS与GWM（0.57）、DSS与QT（0.48）之间存在适度的一致性。寄生虫强度不影响(P >；结果表明，QT是诊断自然感染牛肝梭菌感染最敏感、最可靠的共寄生虫学技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).