Carbapenem resistance gene blaNDM-1 in Escherichia coli, Serratia marcescens and Klebsiella michiganensis strains from a pediatric patient

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-06-06 DOI:10.1016/j.jiph.2025.102869

Yuanyuan Li , Xiangkun Zeng , Mi Zhou , Yi Liu , Chen Xu , Xiaofan Li , Yunbing Li , Lili Huang , Ning Dong

引用次数: 0

Abstract

Carbapenem resistance has a widespread globally. In this study, three bacterial strains including Escherichia coli (SXY-EC), Serratia marcescens (SXY-SM), and Klebsiella michiganensis (SXY-KM) were isolated from a pediatric patient in China. Whole-genome sequencing and resistome analysis revealed the appearance of bla_NDM-1 β-lactamase gene in all these three strains, and conjugation tests confirmed its transferability. Additionally, SXY-SM and SXY-KM harbored two to three other resistance genes, while SXY-EC carried eleven. Notably, the multidrug resistance region containing bla_NDM-1 in plasmid pSXY-EC_NDM1 from SXY-EC was flanked by mobile elements, potentially enabling intermolecular replicative transposition mediated by distinct insertion sequences.

查看原文本刊更多论文

大肠杆菌、粘质沙雷氏菌和密歇根克雷伯氏菌中碳青霉烯耐药基因blaNDM-1的研究

碳青霉烯耐药性在全球范围内广泛存在。本研究从中国一名小儿患者身上分离到大肠埃希菌（SXY-EC）、粘质沙雷菌（SXY-SM）和密歇根克雷伯菌（SXY-KM） 3株细菌。全基因组测序和抗性组分析显示，这3株菌株均存在blaNDM-1 β-内酰胺酶基因，偶联试验证实了其可转移性。此外，SXY-SM和SXY-KM携带2 ~ 3个抗性基因，而SXY-EC携带11个抗性基因。值得注意的是，来自SXY-EC的质粒pSXY-EC_NDM1中含有blaNDM-1的多药耐药区两侧有可移动元件，可能通过不同的插入序列介导分子间复制转位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.