Influence of turning frequency on removal of pathogens and antibiotic resistance genes from full-size sheep manure composting on the Qinghai-Tibet Plateau

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2025-06-30 DOI:10.1016/j.bej.2025.109845

Rui Cai , Qi Xu , Xi Luo , Guangcheng Xiong , Chu Wang , Wenbo Yu , Chuncheng Xu

{"title":"Influence of turning frequency on removal of pathogens and antibiotic resistance genes from full-size sheep manure composting on the Qinghai-Tibet Plateau","authors":"Rui Cai , Qi Xu , Xi Luo , Guangcheng Xiong , Chu Wang , Wenbo Yu , Chuncheng Xu","doi":"10.1016/j.bej.2025.109845","DOIUrl":null,"url":null,"abstract":"<div><div>Effectively removing pathogenic bacteria, viruses, and antibiotic resistance genes (ARGs) in livestock and poultry manure is essential for the safe utilization of these wastes. The aim of this study was to analyze the impacts of turning frequency on the pathogens, antibiotics, and ARGs during sheep manure composting at a practical scale on Qinghai-Tibet Plateau (QTP). This study set up three treatments for turning once every 2 d (T1), 4 d (T2), and 6 d (T3). Results showed that compared with T2 and T3, T1 increased the germination index and humus content by 3.0 %-13.5 % and 1.91 %-7.45 %, respectively. T1 effectively reduced the counts of Coliforms and Salmonella, as well as the abundance of pathogenic bacteria such as Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae. It also decreased viral diversity and abundance. Notably, the removal rates of oxytetracycline, penicillin, cephalosporin C, and tylosin in T1 were increased by 9.14 %, 9.38 %, 12.6 %, and 12.5 %, respectively, compared to T3, and diminished the abundance of ARGs conferring resistance to macrolide-lincosamide-streptogramin, multidrug, bacitracin, tetracycline, and beta-lactam. Jeotgalicoccus, Corynebacterium, Herbinix, and Clostridium were the main hosts of ARGs in raw materials, while Pseudoxanthomonas, Luteimonas, Microbacterium, Alcanivorax, Devosia, and Pusillimonas were the main hosts in compost products. These findings provide a theoretical foundation and technical guidance for the harmless treatment and resourceful utilization of sheep manure on the QTP.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109845"},"PeriodicalIF":3.7000,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X25002190","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Effectively removing pathogenic bacteria, viruses, and antibiotic resistance genes (ARGs) in livestock and poultry manure is essential for the safe utilization of these wastes. The aim of this study was to analyze the impacts of turning frequency on the pathogens, antibiotics, and ARGs during sheep manure composting at a practical scale on Qinghai-Tibet Plateau (QTP). This study set up three treatments for turning once every 2 d (T1), 4 d (T2), and 6 d (T3). Results showed that compared with T2 and T3, T1 increased the germination index and humus content by 3.0 %-13.5 % and 1.91 %-7.45 %, respectively. T1 effectively reduced the counts of Coliforms and Salmonella, as well as the abundance of pathogenic bacteria such as Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae. It also decreased viral diversity and abundance. Notably, the removal rates of oxytetracycline, penicillin, cephalosporin C, and tylosin in T1 were increased by 9.14 %, 9.38 %, 12.6 %, and 12.5 %, respectively, compared to T3, and diminished the abundance of ARGs conferring resistance to macrolide-lincosamide-streptogramin, multidrug, bacitracin, tetracycline, and beta-lactam. Jeotgalicoccus, Corynebacterium, Herbinix, and Clostridium were the main hosts of ARGs in raw materials, while Pseudoxanthomonas, Luteimonas, Microbacterium, Alcanivorax, Devosia, and Pusillimonas were the main hosts in compost products. These findings provide a theoretical foundation and technical guidance for the harmless treatment and resourceful utilization of sheep manure on the QTP.

查看原文本刊更多论文

翻转频率对青藏高原全尺寸羊粪堆肥病原菌和抗生素抗性基因去除的影响

有效去除畜禽粪便中的致病菌、病毒和抗生素耐药基因（ARGs）对于这些废物的安全利用至关重要。本研究旨在分析青藏高原羊粪堆肥过程中翻转频率对病原菌、抗生素和ARGs的影响。本研究设置3个处理，每2 d （T1）、4 d （T2）和6 d （T3）翻转1次。结果表明，与T2和T3处理相比，T1处理的发芽指数和腐殖质含量分别提高了3.0 % ~ 13.5 %和1.91 % ~ 7.45 %。T1有效降低大肠菌群和沙门氏菌的数量，以及金黄色葡萄球菌、鲍曼不动杆菌、肺炎克雷伯菌等致病菌的丰度。它还降低了病毒的多样性和丰度。值得注意的是，与T3相比，T1对土霉素、青霉素、头孢菌素C和泰洛菌素的去除率分别提高了9.14 %、9.38 %、12.6 %和12.5 %，并减少了对大环内酯-林科胺-链霉素、多药、杆菌肽、四环素和β -内酰胺耐药的ARGs的丰度。原料中ARGs的主要寄主是Jeotgalicoccus、棒状杆菌（Corynebacterium）、Herbinix和Clostridium，堆肥产品中ARGs的主要寄主是Pseudoxanthomonas、Luteimonas、Microbacterium、Alcanivorax、Devosia和Pusillimonas。研究结果为羊粪无害化处理和资源化利用提供了理论依据和技术指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.