Caroline Ribeiro Corrêa, Sabrina Carra, Vanderson Antônio de Lima, Camila Klein, João Vitor Faccin Barbosa, Eloane Malvessi
{"title":"Xylonic acid: A novel approach to bioproduction by sustainable strategies","authors":"Caroline Ribeiro Corrêa, Sabrina Carra, Vanderson Antônio de Lima, Camila Klein, João Vitor Faccin Barbosa, Eloane Malvessi","doi":"10.1016/j.bej.2025.109846","DOIUrl":null,"url":null,"abstract":"<div><div>Despite its recognized potential use in polyamides, cosmetic formulations, hydrogels, and as an antimicrobial, the sustainable bioproduction of xylonic acid remains underestimated in the field of industrial biotechnology. In parallel, sorbitol, a high added-value polyol widely used in the food and pharmaceutical industries, can also be obtained through biotechnological routes. Both compounds can be synthesized from xylose and fructose through the coordinated enzymatic action of glucose-fructose oxidoreductase (GFOR) and glucono-δ-lactonase (GL), periplasmic enzymes produced by <em>Zymomonas mobilis</em> cells. In this study, the activity of the GFOR/GL complex was evaluated under different pH (5.8–7.6) and temperature (34–53 °C) conditions, using 700 mmol/L of xylose/fructose as substrates and 4 g/L of biocatalyst. The optimum activity conditions were identified between pH 6.8–7.2 and 47–50 °C. Subsequent bioconversion trials demonstrated impressive xylonic acid yields of 90 % at 39 °C and pH 6.4 using free enzymes, and 89 % at 43 °C and pH 6.4 when enzymes were immobilized in calcium alginate. Reusability tests revealed the immobilized biocatalyst's stable performance over five cycles, exhibiting no significant loss of efficiency in xylonic acid production. These findings underscore the potential of the GFOR/GL system as an efficient and sustainable alternative for valorizing renewable sugars into high-value industrial products.</div></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":"222 ","pages":"Article 109846"},"PeriodicalIF":3.7000,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X25002207","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Despite its recognized potential use in polyamides, cosmetic formulations, hydrogels, and as an antimicrobial, the sustainable bioproduction of xylonic acid remains underestimated in the field of industrial biotechnology. In parallel, sorbitol, a high added-value polyol widely used in the food and pharmaceutical industries, can also be obtained through biotechnological routes. Both compounds can be synthesized from xylose and fructose through the coordinated enzymatic action of glucose-fructose oxidoreductase (GFOR) and glucono-δ-lactonase (GL), periplasmic enzymes produced by Zymomonas mobilis cells. In this study, the activity of the GFOR/GL complex was evaluated under different pH (5.8–7.6) and temperature (34–53 °C) conditions, using 700 mmol/L of xylose/fructose as substrates and 4 g/L of biocatalyst. The optimum activity conditions were identified between pH 6.8–7.2 and 47–50 °C. Subsequent bioconversion trials demonstrated impressive xylonic acid yields of 90 % at 39 °C and pH 6.4 using free enzymes, and 89 % at 43 °C and pH 6.4 when enzymes were immobilized in calcium alginate. Reusability tests revealed the immobilized biocatalyst's stable performance over five cycles, exhibiting no significant loss of efficiency in xylonic acid production. These findings underscore the potential of the GFOR/GL system as an efficient and sustainable alternative for valorizing renewable sugars into high-value industrial products.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.