Antibacterial activity and mechanism of polyketides from the marine-derived fungus Talaromyces sp.

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2025-06-30 DOI:10.1016/j.phytochem.2025.114606

Ruiyun Huo , Guangrong Zi , Dongxiao Shi , Ying Shi , Jinwei Ren , Kui Hong , Ling Liu

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引用次数: 0

Abstract

Six previously undescribed natural products, talaromenes A−D (1−4), prugosene F (5), and prugosene G (6), as well as four previously known compounds (7−10), were isolated and characterized from the culture of the marine-derived fungus Talaromyces sp. WHUF04072. The structures of these compounds were determined by the HRESIMS and NMR spectroscopic data. The absolute configurations of the unreported compounds were determined by ECD calculations and ECD experiments induced by Rh₂(OCOCF₃)₄. Moreover, all isolated compounds were evaluated for their antibacterial activities. Compound 8 exhibit significant antibacterial activities against several strains of Staphylococcus aureus with MICs ranging from 0.125 to 0.25 μg/mL. Additionally, it can effectively inhibit the biofilms formation of methicillin-resistant S. aureus (MRSA). Further mechanism studies revealed that 8 could exhibited significant antibacterial effects against MRSA by disrupting bacterial membranes. These results suggested that 8 may be developed into a new antibacterial candidate for combating MRSA infections.

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海洋源真菌Talaromyces sp.聚酮类化合物的抑菌活性及抑菌机理研究。

从海洋来源的真菌Talaromyces sp. WHUF04072中分离并鉴定了六个先前未被描述的天然产物，talaromenes A−D（1−4）、prugosene F(5)和prugosene G(6)，以及四个先前已知的化合物（7−10）。这些化合物的结构通过hresms和NMR谱数据进行了测定。通过ECD计算和Rh2(OCOCF3)4诱导的ECD实验确定了未报道化合物的绝对构型。此外，对所有分离得到的化合物进行了抗菌活性评价。化合物8对多种金黄色葡萄球菌具有明显的抑菌活性，mic范围为0.125 ~ 0.25 μg/mL。此外，它还能有效抑制耐甲氧西林金黄色葡萄球菌（MRSA）生物膜的形成。进一步的机制研究表明，8可能通过破坏细菌膜而对MRSA表现出明显的抗菌作用。这些结果表明，8可能成为一种新的抗MRSA感染的候选抗菌药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.