Evaluation of the relative gene expression of FGF2, HAS2, BCL2, VEGFA, and PGR in repeat-breeder cows undergoing cyclicity or acyclicity

IF 2.5 3区生物学 Q3 REPRODUCTIVE BIOLOGY

Reproductive biology Pub Date : 2025-07-03 DOI:10.1016/j.repbio.2025.101049

Punnawut Yama , Napatsorn Montha , Jakree Jitjumnong , Hien Van Doan , Nguyen Vu Linh , Warittha U-krit , Maslin Osathanunkul , Raktham Mektrirat , Julakorn Panatuk , Payungsuk Intawicha , Chien-Kai Wang , Tossapol Moonmanee

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引用次数: 0

Abstract

Repeat breeder syndrome (RBS) is one of the main reproductive failures in mono-ovulatory dairy cattle. The differential relative expression of several important genes in the ovary and uterus were evaluated in repeat-breeder dairy cattle. Cows classified as RBS, defined as those that had failed to conceive after at least three consecutive inseminations, with no clinical abnormalities. The selected cows were divided into two groups: repeat-breeder dairy cows undergoing cyclicity (control group) (n = 8) or acyclicity (n = 6). Granulosa cells (GCs) and endometrium were collected from both groups to evaluate the relative expression levels of target genes (fibroblast growth factor 2 [FGF2], hyaluronan synthase 2 [HAS2], B-cell lymphoma 2 [BCL2], vascular endothelial growth factor A [VEGFA], and progesterone receptor [PGR]). The relative expression levels of FGF2 mRNA in GCs (0.34-fold and –65.84 %) and PGR mRNA in endometrium (0.09-fold and –90.53 %) were lower (P < 0.05) in acyclic cows relative to cyclic cows. The relative expression levels of HAS2 and BCL2 mRNA in GCs tended to be lower by 0.40-fold (–59.85 %, P = 0.084) and 0.54-fold (–45.84 %, P = 0.065) in acyclic cows relative to cyclic cows. However, none of the relative expression levels of VEGFA in the ovary and uterus differed between groups (P > 0.10). These results provide a comparison of differential gene expression in repeat-breeder dairy cows undergoing cyclicity and acyclicity, suggesting a role in ovarian follicular growth and uterine function.

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FGF2、HAS2、BCL2、VEGFA和PGR基因在循环和非循环状态下的相对表达评价

重复繁殖综合征（RBS）是单排卵奶牛繁殖失败的主要原因之一。研究了重复种牛卵巢和子宫中几个重要基因的差异相对表达。被归类为RBS的奶牛，定义为在至少连续三次人工授精后未能怀孕，没有临床异常的奶牛。将选取的奶牛分为两组：循环奶牛组（n = 8）和不循环奶牛组（n = 6）。采集两组患者的颗粒细胞（GCs）和子宫内膜，评估靶基因（成纤维细胞生长因子2 [FGF2]、透明质酸合成酶2 [HAS2]、b细胞淋巴瘤2 [BCL2]、血管内皮生长因子A [VEGFA]、孕激素受体[PGR]）的相对表达水平。非循环奶牛GCs中FGF2 mRNA的相对表达量（0.34倍，-65.84 %）和子宫内膜中PGR mRNA的相对表达量（0.09倍，-90.53 %）低于循环奶牛（P <； 0.05）。HAS2和BCL2 mRNA在GCs中的相对表达量相对于循环奶牛有降低0.40倍（-59.85 %,P = 0.084）和0.54倍（-45.84 %,P = 0.065）的趋势。然而，各组间卵巢和子宫中VEGFA的相对表达量均无差异（P >； 0.10）。这些结果提供了在周期性和非周期性重复繁殖奶牛中差异基因表达的比较，提示其在卵巢卵泡生长和子宫功能中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive biology 生物-生殖生物学

CiteScore

3.90

自引率

0.00%

发文量

审稿时长

29 days

期刊介绍： An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn, Poland. Reproductive Biology is an international, peer-reviewed journal covering all aspects of reproduction in vertebrates. The journal invites original research papers, short communications, review articles and commentaries dealing with reproductive physiology, endocrinology, immunology, molecular and cellular biology, receptor studies, animal breeding as well as andrology, embryology, infertility, assisted reproduction and contraception. Papers from both basic and clinical research will be considered.