Penehyclidine Hydrochloride Improves Ischemia-Reperfusion Injury in Lung Transplantation by Inhibiting Inflammatory Response and Oxidative Stress Injury

IF 0.8 4区 医学 Q4 IMMUNOLOGY
Rongfang Liu , Qiaoying Cai , Yongle Li , Xuguang Zhang
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引用次数: 0

Abstract

Objectives

Penehyclidine hydrochloride, a common anticholinergic drug, shows protective effects on ischemia-reperfusion injury (IRI) in various organs. However, the effects of penehyclidine hydrochloride on lung IRI induced by lung transplantation (LTx) have not been known, especially during the cold ischemia phase. Therefore, this study aimed to explore the effect of penehyclidine hydrochloride on lung IRI in a rat model of LTx.

Material and methods

Rats were randomly divided into sham group, control group, and penehyclidine hydrochloride group (PHC group). Rats in the sham group only underwent thoracotomies without LTx. Rats in the C group and PHC group established LTx model and received no treatment or penehyclidine hydrochloride respectively during the cold ischemia phase. At 120 minutes after LTx, the blood gas analysis, wet to dry ration (W/D), inflammatory reaction, oxidative stress injury, and lung structure were detected. Additionally, the type II alveolar epithelial cells observed by transmission electron microscopy were also explored.

Results

Compared with C group, the oxygenation, W/D, inflammatory markers, oxidative stress markers and lung structure in the C group and PHC group deteriorated, which improved in the PHC group compared with those in the C group (P < .05). Additionally, the type II alveolar epithelial cells were also less damaged in the PHC group compared with the C group (P < .05).

Conclusions

Penehyclidine hydrochloride applied during the cold ischemia phase improved lung IRI caused by LTx via inhibiting inflammatory response and oxidative stress injury, and maintained the integrity of organizational structure in the type II alveolar epithelial cells.
盐酸戊乙奎醚通过抑制炎症反应和氧化应激损伤改善肺移植缺血再灌注损伤。
目的:盐酸戊乙基醚是一种常见的抗胆碱能药物,对多器官缺血再灌注损伤(IRI)有保护作用。然而,盐酸戊乙奎醚对肺移植(LTx)诱导的肺IRI的影响尚不清楚,特别是在冷缺血阶段。因此,本研究旨在探讨盐酸戊乙奎醚对LTx大鼠模型肺IRI的影响。材料与方法:将大鼠随机分为假手术组、对照组和盐酸戊乙奎醚组(PHC组)。假手术组大鼠仅行开胸手术,未行LTx。C组和PHC组大鼠分别建立LTx模型,在冷缺血期不给药或不给盐酸戊乙乙胺。LTx后120分钟,检测血气分析、干湿比(W/D)、炎症反应、氧化应激损伤、肺组织结构。透射电镜观察ⅱ型肺泡上皮细胞。结果:与C组比较,C组和PHC组肺氧合、W/D、炎症指标、氧化应激指标、肺结构均恶化,PHC组较C组改善(P < 0.05)。此外,PHC组ⅱ型肺泡上皮细胞损伤程度也低于C组(P < 0.05)。结论:冷缺血期应用盐酸戊乙基醚可通过抑制炎症反应和氧化应激损伤改善LTx所致的肺IRI,维持ⅱ型肺泡上皮细胞组织结构的完整性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Transplantation proceedings
Transplantation proceedings 医学-免疫学
CiteScore
1.70
自引率
0.00%
发文量
502
审稿时长
60 days
期刊介绍: Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.
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