Blood group O expression in normal tissues and tumors.

Ea Kristine Clarisse Tulin, Lukas Muerner, Tanya R McKitrick, Catherine A Tilton, Lauren E Pepi, Tianwei Jia, Maxence Noel, Leon Y Zheng, Sean R Stowell, Stephan von Gunten, Jamie Heimburg-Molinaro, Richard D Cummings
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Abstract

The H antigen (O blood group), the precursor to A and B blood groups, is expressed on human erythrocytes and other cells (e.g., endothelial cells). Remarkably, the specific expression of the H antigens is uncertain due to the lack of specific reagents for its detection. Here, we describe two monoclonal antibodies (mAbs), Tn4-31L and OmcFL3-02, generated by immunizing sea lampreys with human cells. Both mAbs are highly specific in comparison with the lectin Ulex europaeus agglutinin-I (UEA-I). We identified expression of H antigens in mammalian glycoproteins, human cells, and normal and malignant human tissues, discovering that different types of human carcinomas exhibit unexpected H antigen expression. Interestingly, H antigen detection in many cases was distinctly elevated by enzymatic desialylation, as well as being elevated in cells engineered to lack sialic acid. These highly specific mAbs will be valuable reagents in blood testing and for exploring the expression and function of H antigens.

O型血在正常组织及肿瘤中的表达。
H抗原(O血型)是A和B血型的前体,在人红细胞和其他细胞(如内皮细胞)上表达。值得注意的是,由于缺乏检测H抗原的特异性试剂,H抗原的特异性表达是不确定的。在这里,我们描述了两种单克隆抗体(mAbs), Tn4-31L和OmcFL3-02,由人细胞免疫七鳃鳗产生。这两种单克隆抗体与凝集素UEA-I相比具有高度特异性。我们鉴定了H抗原在哺乳动物糖蛋白、人类细胞以及正常和恶性人类组织中的表达,发现不同类型的人类肿瘤表现出意想不到的H抗原表达。有趣的是,在许多情况下,H抗原检测通过酶解去盐化而明显升高,在缺乏唾液酸的细胞中也会升高。这些高特异性单克隆抗体将成为血液检测和探索H抗原表达和功能的宝贵试剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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