High-Risk Human Papilloma Virus Genotyping in Patients with Clinically Suspected Precancerous and Cancerous Lesions of Cervix.

Abstract

Nowadays, human papilloma virus (HPV) testing has been added with cytology to aid in detection of high-grade cervical lesions, especially in women aged ≥30 years. This study aimed to evaluate the relationship between high-risk HPV (hrHPV) genotype and clinicopathological parameters among the diagnosed patients with both precancerous and cancerous cervical lesions. This cross-sectional study was carried out in the Department of Pathology of Dhaka Medical College and in the collaboration with 'DNA Solution LTD, Dhaka', Bangladesh. The study was conducted over a period of 24 months from July 2018 to June 2020. Following informed written consent, a total of 51 women (18 patients with clinically suspected malignant lesions and 33 with a positive VIA test) were included in this study, and all were subjected to colposcopic examination performed by a gynecologist. Ethical issues were ensured in accordance with the 1975 Declaration of Helsinki. SPSS version 24.0 was used during data analysis. The average age ±SD of the patients with cervical lesions was 41.86±9.84 years with a range from 25 to 60 years. Approximately half of the participants (n=25, 49.0%) were married for less than 16 years and almost one-tenth were multiparous. Among all cytological and histological findings, hrHPV-16 (n=21, 41.18%) was commonest, followed by hrHPV-18 (n=3, 5.88%) and hrHPV-68 (n=1, 1.96%). Two patients had co-infection with hrHPV (16, 18) and hrHPV (16, 51) in particular. However, the rest 24 cases remained undetected. Among the hrHPV-positive cases (n=27), hrHPV-16 was detected in 5 cases while those were negative for intraepithelial lesions. In cervical swabs, 6 cases of hrHPV-16, 2 cases of hrHPV-18 and one case of co-infection hrHPV (16, 18) were histologically diagnosed as invasive SCC G-II. Among 51 cases, the most common histological diagnosis was CIN I (n=18, 35.3%). hrHPV genotyping was found to be associated with cytological findings and histological diagnosis.