In Diabetic Aged Female Rats, Resveratrol Supplementation Prevents Retinal Tissue Damage By Increasing Antioxidant Activity Through SIRT1 Gene Expression.

IF 1 4区医学 Q4 CLINICAL NEUROLOGY

Noropsikiyatri Arsivi-Archives of Neuropsychiatry Pub Date : 2025-06-18 eCollection Date: 2025-01-01 DOI:10.29399/npa.28586

Adem Atacak, Haluk Gümüş, Elif Gülbahçe Mutlu, Saltuk Buğra Baltacı, Rasim Moğulkoç, Abdulkerim Kasım Baltacı

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Abstract

Introduction: The present study was carried out to investigate how resveratrol administration affects retinal SIRT1 levels and retinal tissue damage in diabetic elderly female rats.

Methods: A total of 24 elderly female rats were divided equally into 4 groups (G): G1, Control; G2, Control + Resveratrol; G3, Diabetes; G4, Diabetes + Resveratrol. Experimental diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ) in G3 and G4. The G2 and G4 were given intraperitoneal (ip) resveratrol (5 mg/kg/day) for 4 weeks in addition to the normal diet. After 4-weeks of resveratrol treatment, Sirtuin 1 (SIRT1) gene expression and malondialdehyde (MDA) ve glutathione (GSH) levels were determined by PCR and ELISA, respectively, in retinal tissue samples of the animals.

Results: The highest retinal MDA values were in the diabetes group (G3), the highest retinal GSH levels were in the Diabetes + Resveratrol group (G4). The retinal MDA and GSH levels of the other groups were not different from each other. The highest retinal SIRT1 expression values were in the Diabetes + Resveratrol (G4) group. The retinal SIRT1 expression values of the diabetes group (G3) were lower than G4, and higher than the G1 and G2. Retinal SIRT1 expression values of the G1 and G2 were not different from each other.

Conclusion: Resveratrol supplementation prevented retinal tissue damage that occurs in diabetic-aged female rats. This antidiabetic effect of resveratrol supplementation occurs by increasing both antioxidant activity and SIRT1 expression in diabetic-aged rats.

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在糖尿病老年雌性大鼠中，补充白藜芦醇通过增加SIRT1基因表达的抗氧化活性来预防视网膜组织损伤。

本研究旨在探讨白藜芦醇对老年糖尿病雌性大鼠视网膜SIRT1水平及视网膜组织损伤的影响。方法：老龄雌性大鼠24只，随机分为4组(G): G1组、对照组；G2，对照+白藜芦醇；G3、糖尿病;G4，糖尿病+白藜芦醇。小鼠G3、G4腹腔单次注射链脲佐菌素诱导实验性糖尿病。G2和G4在正常饮食的基础上，连续4周腹腔注射白藜芦醇（5 mg/kg/天）。白藜芦醇治疗4周后，分别采用PCR和ELISA法检测小鼠视网膜组织样本中SIRT1 （SIRT1）基因表达和丙二醛（MDA） ve谷胱甘肽（GSH）水平。结果：糖尿病组（G3）视网膜MDA值最高，糖尿病+白藜芦醇组（GSH）视网膜GSH水平最高。各组视网膜丙二醛和谷胱甘肽水平差异无统计学意义。糖尿病+白藜芦醇（G4）组视网膜SIRT1表达值最高。糖尿病组（G3）视网膜SIRT1表达值低于G4，高于G1和G2。G1和G2的视网膜SIRT1表达值无明显差异。结论：补充白藜芦醇可预防糖尿病老龄雌性大鼠视网膜组织损伤。补充白藜芦醇的这种抗糖尿病作用是通过增加糖尿病老龄大鼠的抗氧化活性和SIRT1表达来实现的。

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来源期刊

Noropsikiyatri Arsivi-Archives of Neuropsychiatry CLINICAL NEUROLOGY-

CiteScore

1.70

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Archives of Neuropsychiatry (Arch Neuropsychiatry) is the official journal of the Turkish Neuropsychiatric Society. It is published quarterly, and four editions annually constitute a volume. Archives of Neuropsychiatry is a peer reviewed scientific journal that publishes articles on psychiatry, neurology, and behavioural sciences. Both clinical and basic science contributions are welcomed. Submissions that address topics in the interface of neurology and psychiatry are encouraged. The content covers original research articles, reviews, letters to the editor, and case reports.