Comparative genomic and virulence analyses of a novel sequence type 420 Streptococcus equi subspecies zooepidemicus isolated from donkey.

IF 5.5 1区 农林科学 Q1 IMMUNOLOGY
Virulence Pub Date : 2025-12-01 Epub Date: 2025-06-29 DOI:10.1080/21505594.2025.2525964
Yuhui Tian, Yan Su, Xinyu Jiang, Lingling Su, Baojiang Zhang, Fenfen Lv
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引用次数: 0

Abstract

The zoonotic pathogen Streptococcus equi subspecies zooepidemicus (SEZ) frequently colonizes equines harmlessly but can occasionally cause disease or cross species barriers. Currently, growing evidence suggests SEZ can lead to severe clinical manifestations in horses and other animals, posing a threat to human and companion animal health. In this study, we sequenced the complete genome of the SEZ strain HT321, a novel sequence type 420 isolated from a donkey with a respiratory infection in China. Subsequently, we conducted comparative genomics, core genome single nucleotide polymorphisms (cgSNP), phylogenetic analysis multilocus sequence typing (MLST), and in vitro pathogenic analysis of this isolate. 118 genes in HT321 were annotated as antibiotic resistance genes (ARGs) and comparative genomics revealed that HT321 contained more lincosamide ARGs compared to other strains. The genomic island of HT321 carried more defensive virulence genes than that in the horse strain JMC111. Furthermore, compared to the reference equine strain JMC111, HT321 exhibited superior antimicrobial resistance and biofilm formation capability but lower pathogenicity. Interestingly, core genome single-nucleotide polymorphism phylogenetic analysis of 51 SEZ strains demonstrated that HT321 clustered with horse and donkey SEZ strains as well as S. canis strains. Notably, MLST analysis of the HT321 and 116 SEZ strains indicated that the HT321 donkey strain was related to SEZ canis isolates. These findings provide valuable insights for understanding, tracking, controlling, and preventing diseases caused by SEZ in donkeys.

驴源新序列420型马链球菌亚种的比较基因组学和毒力分析。
人畜共患病原体马链球菌亚种动物流行病(SEZ)经常无害地定殖在马身上,但偶尔也会引起疾病或跨物种障碍。目前,越来越多的证据表明,经济特区可导致马和其他动物出现严重的临床表现,对人类和伴侣动物的健康构成威胁。在这项研究中,我们对SEZ菌株HT321的全基因组进行了测序,这是一种从中国呼吸道感染驴中分离出来的新序列420型。随后,我们对该分离株进行了比较基因组学、核心基因组单核苷酸多态性(cgSNP)、系统发育分析、多位点序列分型(MLST)和体外致病分析。HT321中118个基因被标记为抗生素耐药基因(ARGs),比较基因组学结果显示HT321与其他菌株相比含有更多的lincosamide ARGs。HT321基因组岛携带的防御性毒力基因多于JMC111。此外,与参考马菌株JMC111相比,HT321表现出更强的抗微生物能力和生物膜形成能力,但致病性较低。51株经济特区菌株的核心基因组单核苷酸多态性系统发育分析表明,HT321与马、驴经济特区菌株以及犬链球菌具有聚集性。值得注意的是,HT321和116 SEZ菌株的MLST分析表明,HT321驴株与SEZ犬株有亲缘关系。这些发现为了解、跟踪、控制和预防驴的经济特区引起的疾病提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Virulence
Virulence IMMUNOLOGY-MICROBIOLOGY
CiteScore
9.20
自引率
1.90%
发文量
123
审稿时长
6-12 weeks
期刊介绍: Virulence is a fully open access peer-reviewed journal. All articles will (if accepted) be available for anyone to read anywhere, at any time immediately on publication. Virulence is the first international peer-reviewed journal of its kind to focus exclusively on microbial pathogenicity, the infection process and host-pathogen interactions. To address the new infectious challenges, emerging infectious agents and antimicrobial resistance, there is a clear need for interdisciplinary research.
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