Radiation-induced skin regeneration: A comparative efficacy and safety analysis of alpha, beta, and gamma modalities in murine models.

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-05-01 Epub Date: 2025-05-17 DOI:10.14202/vetworld.2025.1168-1179
Madyan Ahmed Khalaf, Marwan Noori Mohammed, Baida M Ahmed, Sahar A H Al-Sharqi
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引用次数: 0

Abstract

Background and aim: The therapeutic application of ionizing radiation in wound healing, especially with alpha, beta, and gamma modalities, remains largely unexplored despite its potential for enhancing regenerative processes. This study aimed to comparatively analyze the efficacy and safety of alpha radiation (IG-A), beta radiation (IG-B), and gamma radiation (IG-G) modalities in promoting skin regeneration using a murine model of full-thickness excisional wounds.

Materials and methods: Twenty male BALB/c mice were randomized into four groups (n = 5 per group): IG-A, IG-B, IG-G, and an untreated control group (CG). Following surgical induction of full-thickness wounds (8 mm diameter), irradiation groups received 15 min of exposure at four intervals post-surgery using americium-241 (alpha), strontium-91 (beta), and cesium-137 (gamma). Wound healing was monitored macroscopically and microscopically on days 0, 2, 4, 6, 8, and 10. Histological and biochemical assessments included collagen synthesis, epithelialization, neovascularization, and growth factor (vascular endothelial growth factor [VEGF] and platelet-derived growth factor [PDGF]) quantification. Statistical analysis was performed using a one-way analysis of variance.

Results: IG-A significantly accelerated wound healing, achieving approximately 100% wound closure by day 10 compared to 90% and 80% in beta and gamma radiation groups, respectively. Control wounds demonstrated only 38% closure. Histopathological analysis indicated enhanced collagen deposition, neovascularization, sebaceous gland regeneration, and complete epithelialization primarily in the alpha-treated group. Biochemical assays revealed significantly elevated VEGF and PDGF levels in irradiated groups, with IG-A exhibiting the highest expression.

Conclusion: IG-A demonstrated superior efficacy in accelerating wound healing and tissue regeneration compared to beta and gamma modalities. This novel finding suggests a potential therapeutic role for IG-A in clinical wound management strategies.

辐射诱导皮肤再生:小鼠模型中α、β和γ模式的比较疗效和安全性分析。
背景和目的:电离辐射在伤口愈合中的治疗应用,特别是与α, β和γ模式,尽管它有增强再生过程的潜力,但仍在很大程度上未被探索。本研究旨在比较分析α辐射(IG-A)、β辐射(IG-B)和γ辐射(IG-G)三种辐射方式在小鼠全层切除创面模型中促进皮肤再生的有效性和安全性。材料与方法:雄性BALB/c小鼠20只,随机分为IG-A组、IG-B组、IG-G组和未处理对照组(CG),每组5只。手术诱导全层创面(直径8mm)后,照射组术后以镅-241 (α)、锶-91 (β)和铯-137 (γ)进行4次间隔照射,每次15分钟。分别于第0、2、4、6、8、10天在宏观和微观下观察伤口愈合情况。组织学和生化评估包括胶原合成、上皮化、新生血管和生长因子(血管内皮生长因子[VEGF]和血小板源性生长因子[PDGF])的定量。采用单因素方差分析进行统计分析。结果:IG-A显著加速伤口愈合,第10天伤口愈合率约为100%,而β和γ辐射组分别为90%和80%。对照组伤口愈合率仅为38%。组织病理学分析显示,主要在α治疗组,胶原沉积、新生血管、皮脂腺再生和完全上皮化增强。生化分析显示,辐照组VEGF和PDGF水平显著升高,其中IG-A表达最高。结论:与β和γ模式相比,IG-A在加速伤口愈合和组织再生方面表现出优越的疗效。这一新发现提示IG-A在临床伤口管理策略中具有潜在的治疗作用。
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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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