Injectable homologous platelet-rich plasma, alone or in combination with oral omega-3 supplementation, for treating keratoconjunctivitis sicca in dogs.

IF 2 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Veterinary World Pub Date : 2025-05-01 Epub Date: 2025-05-21 DOI:10.14202/vetworld.2025.1262-1273
William Dos Santos Villa, João Victor Goulart Consoni Passareli, Giovana José Garcia Estanho, Marco Aurélio da Cruz Nobre Gomes, Gisele Alborghetti Nai, Cecília Laposy Santarém, Silvia Franco Andrade
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引用次数: 0

Abstract

Background and aim: Keratoconjunctivitis sicca (KCS) is a chronic inflammatory condition of the canine ocular surface primarily caused by immune-mediated destruction of lacrimal tissues. Platelet-rich plasma (PRP) is known for its regenerative and anti-inflammatory properties, while omega-3 (ω-3) fatty acids possess immunomodulatory effects. This study aimed to evaluate the efficacy of injectable homologous PRP (HPRP), alone or in combination with oral ω-3 supplementation, in improving clinical and histopathological parameters in dogs with KCS.

Materials and methods: Twenty-two dogs (44 eyes) with bilateral KCS were randomized into two treatment groups: HPRP (n = 22 eyes) and HPRP plus oral ω-3 (HPRPO; n = 22 eyes). Treatments were administered monthly for up to three sessions alongside topical lubricants. Ophthalmological evaluations - including Schirmer's tear test-1 (STT-1), tear film breakup time (TBUT), fluorescein staining, cytology of the third eyelid gland, and conjunctival histopathology - were performed at baseline and at monthly intervals up to 6 months.

Results: Both groups demonstrated significant improvement in ocular clinical signs and tear secretion. STT-1 values significantly increased from baseline in both groups (p < 0.05), without significant intergroup differences. However, TBUT values were significantly higher in the HPRPO group from month 3 onwards (p < 0.05). Cytological and histological analyses revealed a significant reduction in lymphocyte and neutrophil counts and an increase in goblet cell numbers in both groups, with greater improvement in the HPRPO group (p < 0.05). Earlier resolution of corneal ulcers and reduced ocular inflammation were observed in the HPRPO group.

Conclusion: Injectable HPRP, particularly when combined with oral ω-3 supplementation, is an effective therapeutic modality for managing KCS in dogs. The combination therapy enhanced tear film stability, reduced ocular inflammation, and promoted epithelial repair more effectively than HPRP alone. These findings support the synergistic effect of ω-3 fatty acids with PRP in ocular surface restoration.

可注射同源富血小板血浆,单独或与口服omega-3补充剂联合,用于治疗犬镰状角膜结膜炎。
背景和目的:干燥性角膜结膜炎(KCS)是犬眼表的一种慢性炎症,主要由免疫介导的泪液组织破坏引起。富血小板血浆(PRP)以其再生和抗炎特性而闻名,而omega-3 (ω-3)脂肪酸具有免疫调节作用。本研究旨在评价可注射同源PRP (HPRP)单独或联合口服ω-3补充剂对改善KCS犬临床和组织病理学参数的效果。材料与方法:将22只双侧KCS犬(44眼)随机分为HPRP治疗组(n = 22眼)和HPRP联合口服ω-3 (HPRPO;N = 22只眼)。治疗每月进行三次,同时使用局部润滑剂。眼科评估包括Schirmer泪液试验-1 (STT-1)、泪膜破裂时间(TBUT)、荧光素染色、第三眼睑腺细胞学和结膜组织病理学-在基线和每月间隔长达6个月。结果:两组患者眼部临床症状及泪液分泌均有明显改善。两组STT-1值均较基线显著升高(p < 0.05),组间差异无统计学意义。而HPRPO组TBUT值自第3个月起显著升高(p < 0.05)。细胞学和组织学分析显示,两组患者淋巴细胞和中性粒细胞计数显著减少,杯状细胞计数显著增加,HPRPO组改善更大(p < 0.05)。观察到HPRPO组角膜溃疡的早期消退和眼部炎症的减轻。结论:注射HPRP,特别是与口服ω-3补充剂联合使用,是治疗犬KCS的有效治疗方式。联合治疗增强泪膜稳定性,减少眼部炎症,促进上皮修复比单独HPRP更有效。这些结果支持ω-3脂肪酸与PRP在眼表修复中的协同作用。
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来源期刊
Veterinary World
Veterinary World Multiple-
CiteScore
3.60
自引率
12.50%
发文量
317
审稿时长
16 weeks
期刊介绍: Veterinary World publishes high quality papers focusing on Veterinary and Animal Science. The fields of study are bacteriology, parasitology, pathology, virology, immunology, mycology, public health, biotechnology, meat science, fish diseases, nutrition, gynecology, genetics, wildlife, laboratory animals, animal models of human infections, prion diseases and epidemiology. Studies on zoonotic and emerging infections are highly appreciated. Review articles are highly appreciated. All articles published by Veterinary World are made freely and permanently accessible online. All articles to Veterinary World are posted online immediately as they are ready for publication.
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