Yuming Wang, Hu Zhang, Ning Cao, Bingqian Qi, Feng Zhao, Jingjing Xie
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引用次数: 0
Abstract
The objective of this study was to evaluate the effect of coating on the recovery rate (RR) of phytase activity during the steam conditioning-pelleting (SCP) process. A split-plot design was employed, with phytases assigned to the main plot and two conditioning temperatures (75 or 85 °C) assigned to the subplot. The whole plot was repeated four times. In Exp. 1, six phytases were analyzed, including an uncoated phytase (NP) NP1 (NP1), two coated phytases (CP) CP1 and CP2 derived from NP1, and three commercial phytases (MP) MP1-MP3. In Exp. 2, coating technology was refined based on the results of Exp. 1, and nine phytases were analyzed, including an NP2, five coated phytases CP3-CP7 derived from NP2, and three commercial phytases MP4-MP6. Phytase activity after the steam-conditioning, pelleting, and cooling process was analyzed, and the RR of phytase activity was calculated for each process. In Exp. 1, significant interactions between phytase and conditioning temperature on the RR of phytase activity were observed (P < 0.05). The RR of CP1 and CP2 did not differ from that of NP1. Commercial phytase MP3 exhibited a lower RR than the other four phytases when conditioned at 75 °C (P < 0.05). Except for MP3, the RR of phytases decreased as the conditioning temperature increased (P < 0.05). In Exp. 2, the RR of phytase decreased as the conditioning temperature increased from 75 to 85 °C (P < 0.05). Compared with NP2, the RR increased, and the loss rate of activity for all five coated phytase (CP3-CP7) decreased after the conditioning process (P < 0.05). Commercial phytase MP4 and MP6 had comparable RR to NP2, while MP5 exhibited a comparable RR to CP3-CP7. In conclusion, the coating technology used in Exp. 1 did not increase the RR of phytase during the pelleting process, whereas the improved coating process employed in Exp. 2 effectively increased the thermostability of phytase.
本研究的目的是评价包衣对蒸汽调理制粒（SCP）过程中植酸酶活性回收率（RR）的影响。采用分块设计，将植酸酶分配给主区，将两个调节温度（75或85°C）分配给副区。整个情节重复了四次。实验1分析了6种植酸酶，包括1种未包被植酸酶NP1 （NP1）、2种由NP1衍生的包被植酸酶CP1和CP2以及3种商品植酸酶MP1-MP3。实验2在实验1的基础上改进了包被技术，分析了9种植酸酶，包括1种NP2、5种由NP2衍生的包被植酸酶CP3-CP7和3种商业化植酸酶MP4-MP6。分析了蒸调、制粒和冷却工艺后的植酸酶活性，并计算了各工艺过程中植酸酶活性的相对危险度。在实验1中，植酸酶和调节温度对植酸酶活性的RR有显著的交互作用(P P P P P P
期刊介绍：
Translational Animal Science (TAS) is the first open access-open review animal science journal, encompassing a broad scope of research topics in animal science. TAS focuses on translating basic science to innovation, and validation of these innovations by various segments of the allied animal industry. Readers of TAS will typically represent education, industry, and government, including research, teaching, administration, extension, management, quality assurance, product development, and technical services. Those interested in TAS typically include animal breeders, economists, embryologists, engineers, food scientists, geneticists, microbiologists, nutritionists, veterinarians, physiologists, processors, public health professionals, and others with an interest in animal production and applied aspects of animal sciences.
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