In-vitro and In-silico evaluation of the inhibitory effects of Persea americana leaf extract against calcium oxalate stones.

IF 2.2 2区医学 Q2 UROLOGY & NEPHROLOGY

Urolithiasis Pub Date : 2025-06-30 DOI:10.1007/s00240-025-01791-4

Emel Akyol, Merve Danisman, Mualla Oner

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Abstract

This study investigated the effects of Persea americana extract (PAE) as a potential inhibitor of calcium oxalate monohydrate growth using both in-vitro and in-silico methods with spontaneous crystallization to evaluate its effectiveness in preventing kidney stones. X-ray diffraction was used to characterize the morphology of calcium oxalate (CaOx) crystals, while scanning electron microscopy (SEM) was used to determine their particle size and crystal formation patterns. SwissADME (Absorption, Distribution, Metabolism, Excretion) computational analysis predicted the biological activity of the extract. The experimental results show that the inhibition of crystal growth increases with the amount of PAE in solution, and crystal growth was almost completely inhibited for 450 min at a concentration of 100 mL of PAE. The in vitro results also revealed that Persea americana leaf promoted the formation of calcium oxalate dihydrate (COD) crystals rather than monohydrate crystals. These findings on PAE's inhibitory effect on calcium oxalate crystallization suggest that natural additives could be valuable in treating kidney stone disease.

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美洲美洲叶提取物对草酸钙结石抑制作用的体外和体内评价。

本研究研究了美洲波斯提取物（PAE）作为一种潜在的草酸钙一水生长抑制剂的作用，采用体外和自发结晶的硅法来评估其预防肾结石的有效性。x射线衍射表征了草酸钙（CaOx）晶体的形貌，扫描电镜（SEM）测定了草酸钙晶体的粒径和晶体形成模式。SwissADME（吸收、分布、代谢、排泄）计算分析预测了提取物的生物活性。实验结果表明，PAE对晶体生长的抑制作用随着溶液中PAE用量的增加而增强，当PAE浓度为100 mL时，晶体生长在450min时几乎被完全抑制。体外实验结果还表明，美洲紫花叶促进草酸钙二水合物（COD）结晶的形成，而不是单水结晶的形成。这些关于PAE对草酸钙结晶抑制作用的发现提示天然添加剂在治疗肾结石疾病中可能有价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Urolithiasis UROLOGY & NEPHROLOGY-

CiteScore

4.50

自引率

6.50%

发文量

期刊介绍： Official Journal of the International Urolithiasis Society The journal aims to publish original articles in the fields of clinical and experimental investigation only within the sphere of urolithiasis and its related areas of research. The journal covers all aspects of urolithiasis research including the diagnosis, epidemiology, pathogenesis, genetics, clinical biochemistry, open and non-invasive surgical intervention, nephrological investigation, chemistry and prophylaxis of the disorder. The Editor welcomes contributions on topics of interest to urologists, nephrologists, radiologists, clinical biochemists, epidemiologists, nutritionists, basic scientists and nurses working in that field. Contributions may be submitted as full-length articles or as rapid communications in the form of Letters to the Editor. Articles should be original and should contain important new findings from carefully conducted studies designed to produce statistically significant data. Please note that we no longer publish articles classified as Case Reports. Editorials and review articles may be published by invitation from the Editorial Board. All submissions are peer-reviewed. Through an electronic system for the submission and review of manuscripts, the Editor and Associate Editors aim to make publication accessible as quickly as possible to a large number of readers throughout the world.