QTL mapping and transcriptome analysis identified BnaA03.XTH4 as a novel negative regulator of plant height in Brassica napus L.

IF 4.2 1区 农林科学 Q1 AGRONOMY
Yu Xu, Ning Ma, Zhiting Guo, Xia Liu, Bingbing Zhang, Xiang Liu, Yiji Shi, Mengfan Qin, Yunlin Zhu, Yan Zhang, Yiming Feng, Aixia Xu, Keqi Li, Zhen Huang
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Abstract

Key message: Identified and validated BnaA03.XTH4 as a negative regulator of plant height through QTL mapping and RNA-seq, with promoter structural variation influencing expression, providing a foundation for dwarf breeding in B. napus. Rapeseed (Brassica napus L.) is an important oilseed crop that faces yield limitations due to lodging susceptibility. Plant height (PH) significantly affects seed yield and lodging resistance; however, the genetic mechanisms remain largely elusive. In this study, we developed multiple genetic populations derived from a dwarf line DZ and a tall line GW. Through simple sequence repeat mapping and specific-locus amplified fragment sequencing, we identified twenty-three preliminary quantitative trait loci, explaining 0.82%-27.22% of the phenotypic variation. Integrated analysis pinpointed four major intervals, PHA02, PHA03, PHA09, and PHC04. RNA sequencing analysis revealed differential expression of BnaA03.XTH4, encoding a xyloglucan endotransglucosylase/hydrolase, in DZ versus GW lines. Promoter analysis uncovered a structural variation associated with enhanced transcriptional activity of BnaA03.XTH4 in dwarf lines. Subcellular localization confirmed BnaA03.XTH4 localizes to the cell wall and apoplast. Heterologous overexpression in Arabidopsis thaliana demonstrated a dual function of BnaA03.XTH4, as a negative regulator of PH and stress response. These findings offer new insights into the regulatory mechanisms of PH and offer molecular targets for improving rapeseed breeding strategies.

QTL定位和转录组分析鉴定出BnaA03。XTH4作为甘蓝型油菜株高负调控因子的研究。
关键信息:识别并验证了BnaA03。通过QTL定位和RNA-seq分析,XTH4作为植株高度的负调控因子,启动子结构变化影响其表达,为甘蓝型油菜矮化育种提供基础。油菜(Brassica napus L.)是一种重要的油料作物,由于易倒伏而面临产量限制。株高(PH)显著影响种子产量和抗倒伏能力;然而,遗传机制在很大程度上仍然难以捉摸。本研究以矮系DZ和高系GW为材料,构建了多个遗传群体。通过简单的序列重复定位和特异位点扩增片段测序,初步鉴定出23个数量性状位点,解释了0.82% ~ 27.22%的表型变异。综合分析确定了四个主要区间:PHA02、PHA03、PHA09和PHC04。RNA测序分析显示BnaA03的差异表达。XTH4在DZ和GW系中编码木葡聚糖内转葡萄糖酶/水解酶。启动子分析揭示了与BnaA03转录活性增强相关的结构变异。矮系中的XTH4。亚细胞定位确认为BnaA03。XTH4定位于细胞壁和外质体。BnaA03在拟南芥中的异源过表达表明其具有双重功能。XTH4,作为PH和胁迫反应的负调节因子。这些发现为研究PH的调控机制提供了新的思路,并为改进油菜育种策略提供了分子靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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