{"title":"RNA sequencing of <i>Mycoplasma bovis</i> infecting bovine mammary epithelial cells and bovine mononuclear cells.","authors":"Noriko Imaizumi, Satoshi Gondaira, Tomochika Sugiura, Ayako Eguchi, Koji Nishi, Jumpei Fujiki, Hidetomo Iwano, Hidetoshi Higuchi","doi":"10.4142/jvs.24347","DOIUrl":null,"url":null,"abstract":"<p><strong>Importance: </strong><i>Mycoplasma bovis</i> is the major pathogen of bovine mycoplasmosis, such as mastitis, pneumonia, otitis media, and arthritis. Understanding the pathophysiology of mycoplasmal infections from the pathogen-side is as important as elucidating the host-side effects; however, few studies have focused on the variations in the expression of <i>M. bovis</i> genes.</p><p><strong>Objective: </strong>In this study, we performed RNA sequencing of <i>M. bovis</i> infecting bovine mammary epithelial cells (bMECs) and mononuclear cells (MNCs) to elucidate pathogen-side factors involved in the pathophysiology of <i>M. bovis</i> mastitis.</p><p><strong>Methods: </strong><i>M. bovis</i> was cocultured with bMEC and MNC using a transwell system, and total RNA was extracted for RNA sequencing analysis.</p><p><strong>Results: </strong>We identified 162 differentially expressed genes (DEGs), 104 upregulated and 58 downregulated, in <i>M. bovis</i> infecting bMEC, whereas 220 DEGs, 154 upregulated and 66 downregulated, in <i>M. bovis</i> infecting MNC. Enrichment analysis of upregulated DEGs in <i>M. bovis</i> infecting bMEC revealed multiple pathways with overlapping genes involved in lipid metabolism. The expression of six virulence genes were significantly upregulated in <i>M. bovis</i> infecting bMEC and MNC, whereas the expression of three virulence genes were significantly downregulated in <i>M. bovis</i> infecting MNC.</p><p><strong>Conclusions and relevance: </strong>Our findings indicate that <i>M. bovis</i> exhibits infecting cell-specific gene expression, and its virulence varies with the infecting cell type.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4142/jvs.24347","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Importance: Mycoplasma bovis is the major pathogen of bovine mycoplasmosis, such as mastitis, pneumonia, otitis media, and arthritis. Understanding the pathophysiology of mycoplasmal infections from the pathogen-side is as important as elucidating the host-side effects; however, few studies have focused on the variations in the expression of M. bovis genes.
Objective: In this study, we performed RNA sequencing of M. bovis infecting bovine mammary epithelial cells (bMECs) and mononuclear cells (MNCs) to elucidate pathogen-side factors involved in the pathophysiology of M. bovis mastitis.
Methods: M. bovis was cocultured with bMEC and MNC using a transwell system, and total RNA was extracted for RNA sequencing analysis.
Results: We identified 162 differentially expressed genes (DEGs), 104 upregulated and 58 downregulated, in M. bovis infecting bMEC, whereas 220 DEGs, 154 upregulated and 66 downregulated, in M. bovis infecting MNC. Enrichment analysis of upregulated DEGs in M. bovis infecting bMEC revealed multiple pathways with overlapping genes involved in lipid metabolism. The expression of six virulence genes were significantly upregulated in M. bovis infecting bMEC and MNC, whereas the expression of three virulence genes were significantly downregulated in M. bovis infecting MNC.
Conclusions and relevance: Our findings indicate that M. bovis exhibits infecting cell-specific gene expression, and its virulence varies with the infecting cell type.
期刊介绍:
The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.