RNA sequencing of Mycoplasma bovis infecting bovine mammary epithelial cells and bovine mononuclear cells.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Noriko Imaizumi, Satoshi Gondaira, Tomochika Sugiura, Ayako Eguchi, Koji Nishi, Jumpei Fujiki, Hidetomo Iwano, Hidetoshi Higuchi
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引用次数: 0

Abstract

Importance: Mycoplasma bovis is the major pathogen of bovine mycoplasmosis, such as mastitis, pneumonia, otitis media, and arthritis. Understanding the pathophysiology of mycoplasmal infections from the pathogen-side is as important as elucidating the host-side effects; however, few studies have focused on the variations in the expression of M. bovis genes.

Objective: In this study, we performed RNA sequencing of M. bovis infecting bovine mammary epithelial cells (bMECs) and mononuclear cells (MNCs) to elucidate pathogen-side factors involved in the pathophysiology of M. bovis mastitis.

Methods: M. bovis was cocultured with bMEC and MNC using a transwell system, and total RNA was extracted for RNA sequencing analysis.

Results: We identified 162 differentially expressed genes (DEGs), 104 upregulated and 58 downregulated, in M. bovis infecting bMEC, whereas 220 DEGs, 154 upregulated and 66 downregulated, in M. bovis infecting MNC. Enrichment analysis of upregulated DEGs in M. bovis infecting bMEC revealed multiple pathways with overlapping genes involved in lipid metabolism. The expression of six virulence genes were significantly upregulated in M. bovis infecting bMEC and MNC, whereas the expression of three virulence genes were significantly downregulated in M. bovis infecting MNC.

Conclusions and relevance: Our findings indicate that M. bovis exhibits infecting cell-specific gene expression, and its virulence varies with the infecting cell type.

牛支原体感染牛乳腺上皮细胞和牛单核细胞的RNA测序。
重要性:牛支原体是牛支原体病的主要病原体,如乳腺炎、肺炎、中耳炎和关节炎。从病原体方面了解支原体感染的病理生理与阐明宿主副作用同样重要;然而,很少有研究关注牛分枝杆菌基因表达的变化。目的:通过对牛支原体感染牛乳腺上皮细胞(bmec)和单核细胞(MNCs)的RNA测序,研究牛支原体乳腺炎的病理生理机制。方法:采用transwell系统将牛分枝杆菌与bMEC和MNC共培养,提取总RNA进行RNA测序分析。结果:我们在感染bMEC的牛分枝杆菌中鉴定出162个差异表达基因(DEGs),其中104个上调,58个下调,而在感染MNC的牛分枝杆菌中鉴定出220个差异表达基因(DEGs), 154个上调,66个下调。对感染bMEC的牛分枝杆菌中上调的deg进行富集分析,揭示了参与脂质代谢的多种基因重叠途径。感染bMEC和MNC的牛分枝杆菌中,6个毒力基因的表达显著上调,而感染MNC的牛分枝杆菌中,3个毒力基因的表达显著下调。结论和相关性:我们的研究结果表明,牛分枝杆菌表现出感染细胞特异性基因表达,其毒力随感染细胞类型而变化。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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