Baicalin ameliorated neurological damage in animal models of stroke through its antioxidant and anti-apoptotic effects.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Hyun-Kyoung Son, Dong-Ju Park, Ju-Bin Kang, Phil-Ok Koh
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引用次数: 0

Abstract

Importance: Ischemic stroke leads to neuronal cell death due to a lack of oxygen and glucose. Baicalin is a flavonoid that has antioxidant and anti-inflammatory properties.

Objective: The aim of this study is to elucidate the anti-oxidant and anti-apoptotic effects of baicalin in animal models of stroke.

Methods: Vehicle or baicalin (100 mg/kg) was administered intraperitoneally immediately after the middle cerebral artery occlusion (MCAO) surgery. Neurobehavioral tests were conducted 24 h post-MCAO and brain tissue was isolated to assess histopathological changes and apoptosis-associated protein expression. Additionally, reactive oxygen species (ROS) and lipid peroxidation (LPO) assays were performed to evaluate oxidative stress.

Results: MCAO animals exhibited severe neurological deficits, which were significantly alleviated by baicalin treatment. Baicalin mitigated the up-regulation in ROS and LPO levels induced by surgery. MCAO damage led to severe histopathological lesions and an increase in terminal deoxynucleotidyl transferase dUTP nick end labeling-positive reactions, these alterations were alleviated by baicalin treatment. MCAO damage decreases the expression of Bcl-2 and increases the expression of Bax, baicalin alleviates these changes. Baicalin also attenuated the upregulation of caspase-3 expression caused by MCAO injury.

Conclusions and relevance: These results can suggest evidence that baicalin exerts neuroprotective effects by preventing apoptosis during cerebral ischemia. In conclusion, baicalin acts as a potent neuroprotective agent through its antioxidant and anti-apoptotic effects on neuronal cell damage.

黄芩苷通过其抗氧化和抗凋亡作用改善脑卒中动物模型的神经损伤。
重要性:缺血性中风由于缺氧和葡萄糖导致神经元细胞死亡。黄芩苷是一种黄酮类化合物,具有抗氧化和抗炎特性。目的:探讨黄芩苷对脑卒中动物模型的抗氧化和抗凋亡作用。方法:大脑中动脉闭塞术后立即腹腔注射黄芩苷或黄芩苷(100 mg/kg)。mcao后24小时进行神经行为测试,并分离脑组织,评估组织病理学变化和凋亡相关蛋白表达。此外,通过活性氧(ROS)和脂质过氧化(LPO)测定来评估氧化应激。结果:MCAO动物表现出严重的神经功能缺损,黄芩苷治疗可显著缓解。黄芩苷可减轻手术引起的ROS和LPO水平上调。MCAO损伤导致严重的组织病理病变,末端脱氧核苷酸转移酶dUTP缺口末端标记阳性反应增加,黄芩苷治疗可减轻这些改变。MCAO损伤可降低Bcl-2的表达,增加Bax的表达,黄芩苷可缓解这一变化。黄芩苷还能减弱MCAO损伤引起的caspase-3表达上调。结论及意义:黄芩苷可能通过抑制脑缺血后细胞凋亡发挥神经保护作用。综上所述,黄芩苷对神经细胞损伤具有抗氧化和抗凋亡作用,是一种有效的神经保护剂。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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