Mustakin Ahmed Shohel, Md Humayan Kabir, Ivvala Anand Shaker, Sumaiya Siddika, Sheikh Arafat Rahman, Sumaiya Siddiquea, Nirmal Chandra Mahat, Tarique Muhammad Tawabul Islam, Md Abul Kashem Tang
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引用次数: 0
Abstract
Objectives: Diarrheal diseases remain a significant global health concern, with rising resistance to traditional pharmaceutical treatments. Litchi chinensis, a tropical fruit, is known for its medicinal properties, but its seed, which is often discarded as waste, has not been extensively studied for its potential health benefits. This study investigates the antidiarrheal effects of L. chinensis seed extract (LCSE).
Methods: Ethanolic extract was prepared from dried, powdered L. chinensis seeds. Acute oral toxicity was conducted by administering a single 2,000 mg/kg dose to female Swiss albino mice. Mice were divided into five groups (control, positive control with Loperamide, and test groups receiving 100, 200, and 400 mg/kg of LCSE) for the antidiarrheal assay. Diarrhea was induced with castor oil, and parameters such as onset of diarrhea, fecal output, gastrointestinal motility, and enteropooling were measured. The statistical analysis was conducted in GraphPad Prism version 10.1.2 using one-way ANOVA followed by a Dunnett multiple comparison test.
Results: L. chinensis seed extract (LCSE) significantly (p < 0.001) delayed the onset of diarrhea, reduced the frequency of defecation, and decreased fecal weight in the castor oil-induced diarrhea model at all doses (100, 200, and 400 mg/kg). Additionally, LCSE significantly (p < 0.001) reduced total and wet fecal output, inhibited gastrointestinal motility (p < 0.001) in the charcoal meal test, and notably (p < 0.001) decreased the volume and weight of intestinal contents in the enteropooling assay. The in vivo antidiarrheal index (ADI) showed a dose-dependent increase, with the highest ADI recorded at 400 mg/kg (189.42%), slightly surpassing the standard drug, loperamide (160.14%).
Conclusion: The finding supports the antidiarrheal potential of LCSE holds potent antidiarrheal properties, positioning it as a promising, sustainable alternative to modern medications.
期刊介绍：
The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.
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