Evaluation of In Vitro Pharmacological Activities and HRLC-MS Based Profiling of Phlogacanthus jenkinsii C. B. Clarke.

IF 1.2 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Pharmacopuncture Pub Date : 2025-06-30 DOI:10.3831/KPI.2025.28.2.116

Sajidul Hoque Ansari, Saikat Sen

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引用次数: 0

Abstract

Objectives: Phlogacanthus jenkinsii is an ethnomedicinal plant used by the people of the North East Region of India to manage various health issues including diabetes and rheumatism. The present study examines the total phenolic and flavonoid content, in vitro pharmacological evaluation of the plant extract/fractions, and phytochemical characterization using HRLC-MS.

Methods: Ethanol extract of Phlogacanthus jenkinsii leaves (PJEE) and its fractions were estimated for total phenolic and flavonoid content following the Folin-Ciocalteu and aluminium chloride method. DPPH (2,2-diphenyl-1-picrylhydrazyl) and NO (nitric oxide) scavenging methods were utilized for evaluating antioxidant properties; albumin denaturation and proteinase inhibitory assay methods for assessing antiinflammatory potential. Additionally, extract/fractions were evaluated for their antidiabetic potential using α-glucosidase and α-amylase inhibition assays. HRLC-MS was employed for phytochemical profiling of the ethyl acetate fraction (PJEAF).

Results: PJEAF contains the highest total phenolic (48.3 ± 0.64 mg GAE/g) and total flavonoid (22.6 ± 0.09 mg QE/g) content. PJEAF scavenges DPPH radical (IC₅₀ = 29.1 ± 0.25 µg/mL) and NO radical (IC₅₀ = 46.6 ± 0.20 µg/mL) potently. PJEAF also exhibited potent antiinflammatory potential with IC₅₀ of 220.8 ± 1.33 and 231.5 ± 1.25 µg/mL against albumin denaturation and protease inhibition assay, respectively. PJEAF had potent inhibition of α-amylase and α-glucosidase enzymes, with IC₅₀ values of 205.9 ± 1.12 and 132.2 ± 0.65 µg/mL, respectively. The HRLC-MS profiling of PJEAF indicated the presence of forty-three phytoconstituents, including 2'-Apo-beta-carotenal, Sinensetin, Kaempferol-3-o-(6"-malonyl-glucoside), Heliannone B, Rhoifolin, Quercetin-3-o-rutinoside.

Conclusion: These findings indicated that Phlogacanthus jenkinsii possesses significant antioxidant, antiinflammatory, and antidiabetic potential, and presence of possible metabolites also makes the plant an important source of drug development and therapeutic applications.

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体外药理活性评价及基于HRLC-MS的分析

目的：詹金phlogacanthus jenkinsii是一种民族药用植物，被印度东北地区的人们用来管理各种健康问题，包括糖尿病和风湿病。本研究利用HRLC-MS检测了植物提取物/组分的总酚和类黄酮含量、体外药理评价和植物化学表征。方法：采用福林- ciocalteu法和氯化铝法对金盏花叶乙醇提取物及其各组分的总酚和总黄酮含量进行测定。采用DPPH（2,2-二苯基-1-吡啶肼基）和NO（一氧化氮）清除法评价其抗氧化性能；评估抗炎潜能的白蛋白变性和蛋白酶抑制试验方法。此外，通过α-葡萄糖苷酶和α-淀粉酶抑制试验评估提取物/馏分的降糖潜力。采用HRLC-MS对乙酸乙酯部分（PJEAF）进行植物化学分析。结果：PJEAF中总酚（48.3±0.64 mg QE/g）和总黄酮（22.6±0.09 mg QE/g）含量最高。PJEAF对DPPH自由基（IC50 = 29.1±0.25µg/mL）和NO自由基（IC50 = 46.6±0.20µg/mL）有较强的清除作用。PJEAF对白蛋白变性和蛋白酶抑制的IC50分别为220.8±1.33µg/mL和231.5±1.25µg/mL。PJEAF对α-淀粉酶和α-葡萄糖苷酶具有较强的抑制作用，IC50值分别为205.9±1.12µg/mL和132.2±0.65µg/mL。HRLC-MS谱分析显示，PJEAF中含有43种植物成分，包括2′-载β -胡萝卜素、紫丁香素、山奈酚-3-o-（6′-丙二酰葡萄糖苷）、向日葵酮B、红花素、槲皮素-3-o-芦丁苷。结论：这些研究结果表明，詹金厚棘具有显著的抗氧化、抗炎和降糖潜力，其代谢产物的存在也使其成为药物开发和治疗应用的重要来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacopuncture INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.10

自引率

7.10%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Pharmacopuncture covers a wide range of basic and clinical science research relevant to all aspects of the biotechnology of integrated approaches using both pharmacology and acupuncture therapeutics, including research involving pharmacology, acupuncture studies and pharmacopuncture studies. The subjects are mainly divided into three categories: pharmacology (applied phytomedicine, plant sciences, pharmacology, toxicology, medicinal plants, traditional medicines, herbal medicine, Sasang constitutional medicine, herbal formulae, foods, agricultural technologies, naturopathy, etc.), acupuncture (acupressure, electroacupuncture, laser acupuncture, moxibustion, cupping, etc.), and pharmacopuncture (aqua-acupuncture, meridian pharmacopuncture, eight-principles pharmacopuncture, animal-based pharmacopuncture, mountain ginseng pharmacopuncture, bee venom therapy, needle embedding therapy, implant therapy, etc.). Other categories include chuna treatment, veterinary acupuncture and related animal studies, alternative medicines for treating cancer and cancer-related symptoms, etc. Broader topical coverage on the effects of acupuncture, the medical plants used in traditional and alternative medicine, pharmacological action and other related modalities, such as anthroposophy, homeopathy, ayurveda, bioelectromagnetic therapy, chiropractic, neural therapy and meditation, can be considered to be within the journal’s scope if based on acupoints and meridians. Submissions of original articles, review articles, systematic reviews, case reports, brief reports, opinions, commentaries, medical lectures, letters to the editor, photo-essays, technical notes, and book reviews are encouraged. Providing free access to the full text of all current and archived articles on its website (www.journal.ac), also searchable through a Google Scholar search.