Effect of a New Formulation Bleaching Gel Containing Nanosized β-Calcium Glycerophosphate on Mechanical Properties, Color, and Cytotoxicity Response: In Vitro Study.

IF 3.2 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Giovanna Lima Fortunato, Klaus Rischka, Rafael Antonio de Oliveira Ribeiro, Carlos Alberto de Souza Costa, Alberto Carlos Botazzo Delbem, Emerson Rodrigues de Camargo, Marcelle Danelon
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引用次数: 0

Abstract

Aim: This study aimed to evaluate, in vitro, the effects of fluoride (F) and nano-sized β-calcium glycerophosphate (β-CaGPnano) added to a 35% H2O2 bleaching gel (HP) on the enamel hardness, morphological properties, the color change, trans-amelodentinal diffusion of H2O2, and cytotoxicity.

Methods: Bovine enamel/dentin samples (n = 126) were divided according to the bleaching gel: a) 35% Hydrogen Peroxide (HP); b) HP Blue 35%-FGM-Produtos Odontológicos (HP Blue); c) HP + 0.1% NaF (HP/F); d) HP + 0.25%β-CaGPnano (HP/0.25%β-CaGPnano); e) HP + 0.1% NaF + 0.25%β-CaGPnano (HP/F/0.25%β-CaGPnano); f) HP + 0.5%β-CaGPnano (HP/0.5%β-CaGPnano); g) HP + 0.1% NaF + 0.5%β-CaGPnano (HP/F/0.5%β-CaGPnano). The bleaching gels were applied thrice (40 min/session) at 7-day intervals between each application. Zeta potential (Zp), polydispersity index (PdI), percentage of loss of surface Vickers hardness (%VHN), surface roughness (Ra), color change, enamel surface morphology, trans-amelodentinal penetration of H2O2, and cell viability (MDPC-23 odontoblast-like cells) were determined.

Results: Similar ZP and PdI values were observed for all bleaching gels (p > 0.05). The treatment with HP/F/0.25%β-CaGPnano resulted in a decrease in %VHN by 86.0%, 81.0%, and 66.0% compared to the HP, HP/F, and HP Blue gels (p < 0.05). For Ra, HP and HP Blue were similar (p > 0.05). All groups receiving F or β-CaGPnano showed a significant increase in color change (p < 0.05). The F/0.5%β-CaGPnano resulted in the lowest H2O2 diffusion/oxidative stress (p < 0.05). A significant reduction in cell viability was observed in all bleached groups compared to the NC (p < 0.05). The F and/or β-CaGPnano gels resulted in the highest level of cell viability (p < 0.05).

Conclusion: Within the limitations of the present study, it was concluded that the incorporation of fluoride and nano-sized β-calcium glycerophosphate (F/β-CaGPnano) into the 35% hydrogen peroxide (H2O2) bleaching gel significantly enhanced its whitening efficacy. The modified formulation also reduced enamel mineral loss when compared to the conventional gel. In addition, the presence of F/β-CaGPnano promoted better morphological preservation of dental tissues and resulted in smoother enamel surfaces. Furthermore, the F/0.5%β-CaGPnano formulation effectively reduced the trans-amelodentinal diffusion of H2O2, leading to the lowest peroxide penetration and oxidative stress levels among the tested groups. Finally, the addition of fluoride and/or β-CaGPnano reduced the cytotoxic effects and increased cell viability, demonstrating improved biocompatibility of the bleaching gel.

含有纳米级β-甘油磷酸钙的新型漂白凝胶对机械性能、颜色和细胞毒性反应的影响:体外研究
目的:研究氟化物(F)和纳米级β-甘油磷酸钙(β-CaGPnano)加入到35% H2O2漂白凝胶(HP)中对牙釉质硬度、形态学、颜色变化、H2O2在牙釉质上的扩散和细胞毒性的影响。方法:126份牛牙釉质/牙本质样品按漂白凝胶分为:a) 35%过氧化氢(HP);b) HP Blue 35%- fgm - products Odontológicos (HP Blue);c) HP + 0.1% NaF (HP/F);d) HP + 0.25%β-CaGPnano (HP/0.25%β-CaGPnano);e)惠普+ 0.1%氟化钠+ 0.25%β-CaGPnano (HP / F / 0.25%β-CaGPnano);f) HP + 0.5%β-CaGPnano (HP/0.5%β-CaGPnano);惠普g) + 0.1%氟化钠+ 0.5%β-CaGPnano (HP / F / 0.5%β-CaGPnano)。漂白凝胶每隔7天使用3次(每次40分钟)。测定Zeta电位(Zp)、多分散性指数(PdI)、表面维氏硬度损失百分比(%VHN)、表面粗糙度(Ra)、颜色变化、牙釉质表面形态、H2O2跨釉面渗透和细胞活力(MDPC-23成牙细胞样细胞)。结果:所有漂白凝胶的ZP和PdI值相似(p < 0.05)。与HP、HP/F和HP Blue凝胶相比,HP/F/0.25%β-CaGPnano治疗组的%VHN降低了86.0%、81.0%和66.0% (p 0.05)。结论:在本研究的限制范围内,在35%过氧化氢(H2O2)漂白凝胶中加入氟化物和纳米级β-甘油磷酸钙(F/β-CaGPnano)可显著增强其美白效果。与传统凝胶相比,改良后的配方还减少了牙釉质矿物质的流失。此外,F/β-CaGPnano的存在促进了牙组织的形态保存,使牙釉质表面更光滑。此外,F/0.5%β-CaGPnano配方有效地降低了H2O2的跨角膜扩散,导致过氧化氢渗透和氧化应激水平在测试组中最低。最后,添加氟化物和/或β-CaGPnano降低了细胞毒性作用,提高了细胞活力,表明漂白凝胶的生物相容性得到改善。
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来源期刊
Journal of Esthetic and Restorative Dentistry
Journal of Esthetic and Restorative Dentistry 医学-牙科与口腔外科
CiteScore
6.30
自引率
6.20%
发文量
124
审稿时长
>12 weeks
期刊介绍: The Journal of Esthetic and Restorative Dentistry (JERD) is the longest standing peer-reviewed journal devoted solely to advancing the knowledge and practice of esthetic dentistry. Its goal is to provide the very latest evidence-based information in the realm of contemporary interdisciplinary esthetic dentistry through high quality clinical papers, sound research reports and educational features. The range of topics covered in the journal includes: - Interdisciplinary esthetic concepts - Implants - Conservative adhesive restorations - Tooth Whitening - Prosthodontic materials and techniques - Dental materials - Orthodontic, periodontal and endodontic esthetics - Esthetics related research - Innovations in esthetics
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