TRPV1 Desensitization Abolishes Metabolic Effects of Time-Restricted Feeding in Rats.

IF 2.9 3区 生物学 Q2 BIOLOGY
Nayara A C Horta, Thais S R Cardoso, Paola Fernandes, Flávia M Araújo, Pedro Lucas Caillaux Luciano, Lucas R Drummond, Cândido C Coimbra, Maristela O Poletini
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Abstract

Time-restricted feeding (TRF) can improve metabolic outcomes. Rodents experiencing TRF exhibit an increase in spontaneous locomotor activity before mealtime and show a phase shift in the rhythm of clock gene expression in peripheral organs, particularly in the liver. Because activation of the transient receptor potential vanilloid-1 (TRPV1) channel produces similar beneficial effects on metabolism as TRF, we hypothesized that this channel mediates the metabolic changes induced by TRF. To assess the role of TRPV1 in metabolism and circadian responses, we utilized the agonist resiniferatoxin (RTX), which at a dosage of 20 µg/kg desensitizes TRPV1. After treatment with RTX or its vehicle, adult male rats were exposed to 21 days of TRF during the light phase. RTX-treated rats show some effects of TRF similar to vehicle-treated controls, with increased locomotor activity and body temperature at the beginning of the light phase, decreased body weight gain and food intake relative to ad-libitum-fed controls. However, RTX-treated rats did not show a decrease in VO2 consumption or an improvement in glucose tolerance induced by TRF. In addition, RTX treatment eliminated the temporal changes in the expression of clock genes Per1 and Rev-Erba in the liver as well as leptin blood levels. In addition, RTX abolished the temporal alterations of the Trb3 gene in the liver, which encodes a protein that negatively modulates insulin signaling without affecting the expression of insulin, Pparα, Pck1, G6pc, or other clock genes in the liver. In conclusion, TRPV1 may participate in the TRF-induced alterations in metabolism, most likely through its regulation of the temporal changes in Per1, Rev-Erba, and Trb3 expressions in the liver, along with leptin secretion.

TRPV1脱敏消除限时喂养大鼠的代谢影响。
限时饲喂(TRF)可以改善代谢结果。经历TRF的啮齿动物在餐前表现出自发运动活动的增加,并表现出外周器官(特别是肝脏)时钟基因表达节奏的相移。由于瞬时受体电位香草素-1 (TRPV1)通道的激活对代谢产生与TRF相似的有益作用,我们假设该通道介导了TRF诱导的代谢变化。为了评估TRPV1在代谢和昼夜节律反应中的作用,我们使用了激动剂树脂干扰素(RTX),其剂量为20µg/kg时可使TRPV1脱敏。在RTX或其载体治疗后,成年雄性大鼠在光照期暴露于21天的TRF。rtx处理的大鼠表现出一些TRF效果,类似于载体处理的对照组,在轻期开始时运动活动和体温增加,相对于自由喂养的对照组,体重增加和食物摄入量减少。然而,rtx处理的大鼠没有表现出由TRF引起的VO2消耗减少或葡萄糖耐量改善。此外,RTX治疗消除了肝脏中时钟基因Per1和Rev-Erba表达以及瘦素血水平的时间变化。此外,RTX消除了肝脏中Trb3基因的时间改变,该基因编码一种负向调节胰岛素信号的蛋白质,而不影响肝脏中胰岛素、Pparα、Pck1、G6pc或其他时钟基因的表达。综上所述,TRPV1可能参与了trf诱导的代谢改变,很可能是通过调节肝脏中Per1、Rev-Erba和Trb3表达的时间变化以及瘦素的分泌来实现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.10
自引率
8.60%
发文量
48
审稿时长
>12 weeks
期刊介绍: Journal of Biological Rhythms is the official journal of the Society for Research on Biological Rhythms and offers peer-reviewed original research in all aspects of biological rhythms, using genetic, biochemical, physiological, behavioral, epidemiological & modeling approaches, as well as clinical trials. Emphasis is on circadian and seasonal rhythms, but timely reviews and research on other periodicities are also considered. The journal is a member of the Committee on Publication Ethics (COPE).
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